Herr Prof. Dr.

Bernhard Hauer

Abteilungsleiter Technische Biochemie
Institut für Biochemie und Technische Biochemie
Abteilung Technische Biochemie


+49 711 685-63193
+49 711 685-63196

Allmandring 31
70569 Stuttgart
Raum: 0.303

  1. Demming, R., Hammer, S., Nestl, B., Gergel, S., Fademrecht, S., Pleiss, J., & Hauer, B. (2019). Asymmetric Enzymatic Hydration of Unactivated, Aliphatic Alkenes. Angew Chem Int Ed Engl, 58, 173–177.
  2. Darimont, D., Weissenborn, M., Nebel, B., & Hauer, B. (2018). Modulating proposed electron transfer pathways in P450BM3 led to improved activity and coupling efficiency. Bioelectrochemistry, 119, 119–123.
  3. Kress, Nico, Halder, J., Rapp, L., & Hauer, B. (2018). Unlocked potential of dynamic elements in protein structures: channels and loops. Curr Opin Chem Biol, 47, 109–116.
  4. Fernandes, A., Halder, J., Nestl, B., Hauer, B., Gernaey, K., & Krühne, U. (2018a). Biocatalyst Screening with a twist: Application of oxygen sensors integrated in microchannels for screening whole cell biocatalyst variants. Bioengineering, 5, E30.
  5. Fernandes, A., Halder, J., Nestl, B., Hauer, B., Gernaey, K., & Krühne, U. (2018b). Biocatalyst Screening with a Twist: Application of Oxygen Sensors Integrated in Microchannels for Screening Whole Cell Biocatalyst Variants. Bioengineering (Basel), 5(2), E30.
  6. Halder, J., Nestl, B., & Hauer, B. (2018). Semi-Rational Engineering of the Naphthalene Dioxygenase from Pseudomonas sp. NCIB 9816-4 towards Selective Asymmetric Dihydroxylation. ChemCatChem, 10, 178–182.
  7. Franden, M., Jayakody, L., Li, W., Wagner, N., Cleveland, N., Michener, W., … Beckham, G. (2018). Engineering Pseudomonas putida KT2440 for efficient ethylene glycol utilization. Metab Eng, 48, 197–207.
  8. Demming, R., Fischer, M., Schmid, J., & Hauer, B. (2018). (De)hydratases-recent developments and future perspectives. Curr Opin Chem Biol, 43, 43–50.
  9. Bastian, S., Hammer, S., Kress, N., Nestl, B., & Hauer, B. (2017). Enabling New Selectivities in the Cyclization of Citronellal by Squalene Hopene Cyclase Variants. ChemCatChem, 9, 4364–4368.
  10. Klenk, J., Nebel, B., Porter, J., Kulig, J., Hussain, S., Richter, S., … Hauer, B. (2017). The self-sufficient P450 RhF expressed in a whole cell system selectively catalyses the 5-hydroxylation of diclofenac. Biotechnol J., 12, 1600520.
  11. Kühnel, L., Nestl, B., & Hauer, B. (2017). Enzymatic Addition of Alcohols to Terpenes by Squalene Hopene Cyclase Variants. ChemBioChem, 18, 2222–2225.
  12. Nestl, B., Geinitz, C., Popa, S., Rizek, S., Haselbeck, R., Fischer, M., … Hauer, B. (2017). Structural and functional insights into asymmetric enzymatic dehydration of alkenols. Nat Chem Biol, 13, 275–281.
  13. Otte, Konrad, Maurer, E., Kirtz, M., Grabs, D., Althoff, E., Bartsch, S., … Hauer, B. (2017). Synthesis of sebacic acid using a de novo designed retro-aldolase as key catalyst. ChemCatChem, 9, 1378–1382.
  14. Demming, R., Otte, K., Nestl, B., & Hauer, B. (2017). Optimized Reaction Conditions Enable the Hydration of Non-natural Substrates by the Oleate Hydratase from Elizabethkingia meningoseptica. ChemCatChem, 9, 758–766.
  15. Darimont, D., Weissenborn, M., Nebel, B., & Hauer, B. (2017). Modulating proposed electron transfer pathways in P450BM3 led to improved activity and coupling efficiency. Bioelectrochemistry, 119, 119–123.
  16. Schmid, J., Steiner, L., Fademrecht, S., Pleiss, J., Otte, K., & Hauer, B. (2017). Biocatalytic study of novel oleate hydratases. J Mol Catal B: Enzym, 133, S243–S249.
  17. Kress, N, Rapp, J., & Hauer, B. (2017). Enantioselective Reduction of Citral Isomers in NCR Ene Reductase: Analysis of an Active-Site Mutant Library. ChemBioChem, 18, 717–720.
  18. Notonier, S., Gricman, L., Pleiss, J., & Hauer, B. (2016). Semi-rational protein engineering of CYP153A M.aq.-CPR BM3 for efficient terminal hydroxylation of short to long chain fatty acids. ChemBioChem, 17, 1550–1557.
  19. Syren, P., Henche, S., Eichler, A., Nestl, B., & Hauer, B. (2016). Squalene-hopene cyclases - evolution, dynamics and catalytic scope. Curr. Opin. Struct. Biol., 41, 73–82.
  20. Hammer, S., Syren, P., & Hauer, B. (2016). Substrate Pre-Folding and Water Molecule Organization Matters for Terpene Cyclase Catalyzed Conversion of Unnatural Substrates. Chemistry Select, 1, 3589–3593.
  21. Reich, S., Nestl, B., & Hauer, B. (2016). Loop Grafted Old Yellow Enzymes in the Bienzymatic Cascade Reduction of Allylic Alcohols. ChemBioChem, 17, 561–565.
  22. Wetzl, D., Bolsinger, J., Nestl, B., & Hauer, B. (2016). α-Hydroxylation of Carboxylic Acids Catalyzed by Taurine Dioxygenase. ChemCatChem, 8, 1361–1366.
  23. Löw, S., Löw, I., Weissenborn, M., & Hauer, B. (2016). Enhanced Ene-Reductase Activity through Alteration of Artificial Nicotinamide Cofactor Substituents. ChemCatChem, 8, 911–915.
  24. Gricman, L., Weissenborn, M., Hoffmann, S., Borlinghaus, N., Hauer, B., & Pleiss, J. (2016). Redox Partner Interaction Sites in Cytochrome P450 Monooxygenases: In Silico Analysis and Experimental Validation. ChemistrySelect, 6, 1243–1251.
  25. Llaudet, E., Darimont, D., Samba, R., Matiychyn, I., Stelzle, M., Weissenborn, M., & Hauer, B. (2016). Expanding an Efficient, Electrically Driven and CNT-Tagged P450 System into the Third Dimension: A Nanowired CNT-Containing and Enzyme-Stabilising 3 D Sol-Gel Electrode. ChemBioChem, 17, 1367–1373.
  26. Kirtz, M., Klebensberger, J., Otte, K., Richter, S., & Hauer, B. (2016). Production of ω-hydroxy octanoic acid with Escherichia coli. J Biotechnol, 230, 30–33.
  27. Weissenborn, M., Notonier, S., Lang, S., Otte, K., Herter, S., Turner, N., … Hauer, B. (2016). Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s. ChemCommun, 52, 6158–6161.
  28. Melcher, M., Facey, S., Henkes, T., Subkowski, T., & Hauer, B. (2016). Accelerated nucleation of hydroxyapatite using an engineered hydrophobin fusion protein. Biomacromolecules.
  29. Hoffmann, SM, Danesh-Azari, H.-R., Spandolf, C., Weissenborn, M., Grogan, G., & Hauer, B. (2016). Structure-guided redesign of CYP153AM.aq. for the improved terminal hydroxylation of fatty acids. ChemCatChem, 8, 3234–3239.
  30. Weissenborn, M., Löw, S., Borlinghaus, N., Kuhn, M., Kummer, S., Rami, F., … Hauer, B. (2016). Enzyme-Catalyzed Carbonyl Olefination by the E. coli Protein YfeX in the Absence of Phosphines. ChemCatChem.
  31. Hoffmann, Sara, Weissenborn, M., Gricman, L., Notonier, S., Pleiss, J., & Hauer, B. (2016). The Impact of Linker Length on P450 Fusion Constructs: Activity, Stability and Coupling. ChemCatChem, 8, 1591–1597.
  32. Hinner, L., Wissner, J., Beurer, A., Nebel, B., & Hauer, B. (2016). Homogeneous vinyl ester-based synthesis of different cellulose derivatives in 1-ethyl-3-methyl-imidazolium acetate. Green Chemistry.
  33. Fademrecht, S., Scheller, P., Nestl, B., Hauer, B., & Pleiss, J. (2016). Identification of imine reductase-specific sequence motifs. Proteins, 84, 600–610.
  34. Lenz, M., Scheller, P., Richter, S., Hauer, B., & Nestl, B. (2016). Cultivation and purification of two stereoselective imine reductases from Streptosporangium roseum and Paenibacillus elgii. Protein Expr. Purif.
  35. Moghimian, P., Kilper, S., Srot, V., Rothenstein, D., Facey, S., Hauer, B., … van, A. P. (2016). Phage-assisted assembly of organic–inorganic hybrid bilayers. International Journal of Materials Research.
  36. Reznicek, O., Facey, S., & Hauer, B. (2015). Draft Genome Sequence of a Papaverine-Degrading, Gram-positive Arthrobacter sp., Isolated from Soil Near Hohenheim, Germany. Genome Announcements, 3, e00422-15.
  37. Gally, C., Nestl, B., & Hauer, B. (2015). Engineering Rieske Non-Heme Iron Oxygenases for the Asymmetric Dihydroxylation of Alkenes. Angew. Chem. Int. Ed., 54, 12952–12956.
  38. Lundemo, M., Notonier, S., Striedner, G., Hauer, B., & Woodley, J. (2015). Process limitations of a whole-cell P450 catalyzed reaction using a CYP153A-CPR fusion construct expressed in Escherichia coli. Appl. Microbiol. Biotechnol., 100, 1197–1208.
  39. Simon, O., Klebensberger, J., Mückschel, B., Kleiber, I., Graf, N., Altenbuchner, J., … Pfannstiel, J. (2015). Analysis of the molecular response of Pseudomonas putida KT2440 to the next-generation biofuel n-butanol. J Proteomics, 122, 11–25.
  40. Steudle, A., Nestl, B., Hauer, B., & Stubenrauch, C. (2015). Activity of squalene-hopene cyclases in bicontinuous microemulsions. Colloids and Surfaces B: Biointerfaces, 135, 735–741.
  41. Reznicek, O., Facey, S., de, W. P., Teunissen, A., de, B. J., Nijland, J., … Hauer, B. (2015). Improved xylose uptake in Saccharomyces cerevisiae due to directed evolution of galactose permease Gal2 for sugar co-consumption. Journal of Applied Microbiology, 119, 99–111.
  42. Reznicek, O., Luesken, F., Facey, S., & Hauer, B. (2015). Draft Genome Sequence of Phenylobacterium immobile Strain E (DSM 1986), Isolated from Uncontaminated Soil in Ecuador. Genome Announcements, 3, e00420-15.
  43. Löw, S., Nestl, B., Weissenborn, M., Zepeck, F., & Hauer, B. (2015). Process Investigations on the One-Pot Synthesis of Rifamycin S Avoiding Chlorinated Solvents. Org. Process Res. Dev., 19, 1544–1547.
  44. Sandig, B., Michalek, L., Vlahovic, S., Antonovici, M., Hauer, B., & Buchmeiser, M. (2015). A Monolithic Hybrid Cellulose-2.5-Acetate/Polymer Bioreactor for Biocatalysis under Continuous Liquid-Liquid Conditions Using a Supported Ionic Liquid Phase. Chemistry, 21, 15835–15842.
  45. Scheller, PN, Lenz, M., Hammer, S., Hauer, B., & Nestl, B. (2015). Imine Reductase-Catalyzed Intermolecular Reductive Amination of Aldehydes and Ketones. ChemCatChem, 7, 3239–3242.
  46. Otte, K, & Hauer, B. (2015). Enzyme engineering in the context of novel pathways and products. Curr Opin Biotechnol, 35, 16–22.
  47. Hammer, S., Marjanovic, A., Dominicus, J., Nestl, B., & Hauer, B. (2015). Squalene Hopene Cyclases - Potency as Protonases for Stereoselective Brønsted Acid Catalysis. Nature Chemical Biology, 11, 121–126.
  48. Lonsdale, T., Lauterbach, L., Honda, M. S., Nestl, B., Hauer, B., & Lenz, O. (2015). H2-driven biotransformation of n-octane to Q1 1-octanol by Q2 a recombinant Pseudomonas putida strain co-synthesizing an O2-tolerant hydrogenase and a P450 monooxygenase. Chem. Commun., 51, 16173–16175.
  49. Ploss, M., Facey, S., Bruhn, C., Zemel, L., Hofmann, K., Stark, R., … Hauer, B. (2014). Selection of peptides binding to metallic borides by screening M13 phage display libraries. BMC Biotechnology, 14, 12.
  50. Otte, Konrad, Kittelberg, J., Kirtz, M., Nestl, B., & Hauer, B. (2014). Whole-Cell One-Pot Biosynthesis of Azelaic Acid. ChemCatChem, 6,4, 1003–1009.
  51. Moghimian, P., Srot, V., Rothenstein, D., Facey, S., Harnau, L., Hauer, B., … van, A. P. (2014). Adsorption and Self-Assembly of M13 Phage into Directionally Organized Structures on C and SiO2 Films. Langmuir, 30, 11428–11432.
  52. Subinya, M., Steudle, A., Nestl, B., Nebel, B., Hauer, B., Stubenrauch, C., & Engelskirchen, S. (2014). Physico-chemical aspects of lipase B from Candida antarctica in bicontinuous microemulstions. Langmuir, 30, 2993–3000.
  53. Reich, S., Kress, N., Nestl, B., & Hauer, B. (2014). Variations in the stability of NCR ene reductase by rational enzyme loop modulation. J. Struct. Biol., 185, 228–233.
  54. Nebel, B., Scheps, D., Honda, M. S., Nestl, B., Breuer, M., Wagner, H., … Hauer, B. (2014). Biooxidation of n-butane to 1-butanol by engineered P450 monooxygenase under increased pressure. Journal of Biotechnology, 191, 86–92.
  55. Scheller, Philipp, Fademrecht, S., Hofelzer, S., Pleiss, J., Leipold, F., Turner, N., … Hauer, B. (2014). Enzyme Toolbox: Novel Enantiocomplementary Imine Reductases. ChemBioChem, 15, 2201–2204.
  56. Syren, P., Hammer, S., Classen, B., & Hauer, B. (2014). Entropy is Key to the Formation of Pentacyclic Terpenoids by Enzyme-Catalyzed Polycyclization. Angew. Chem. Int. Ed., 53, 4845–4849.
  57. Nestl, B., Hammer, S., Nebel, B., & Hauer, B. (2014). New Generation of Biocatalysts for Organic Synthesis. Angew. Chem. Int. Ed., 53, 3070–3095.
  58. Nestl, B., & Hauer, B. (2014). Engineering of Flexible Loops in Enzymes. ACS Catalysis, 4, 3201–3211.
  59. Seitz, M., Syren, P., Steiner, L., Klebensberger, J., Nestl, B., & Hauer, B. (2013). Synthesis of heterocyclic terpenoids by promiscuous squalene-hopene cyclases. ChemBioChem, 14, 436–439.
  60. Nestl, B., & Hauer, B. (2013). Protein engineering: chemistry gets the assist. Nat. Chem. Bio., 9, 470–471.
  61. Rothenstein, D., Facey, S., Ploss, M., Hans, P., Melcher, M., Srot, V., … Bill, J. (2013). Mineralisation of gold nanoparticles using tailored M13 phages. Bioinspired, Biomimetic and Nanobiomaterials.
  62. Otte, Konrad, Kirtz, M., Nestl, B., & Hauer, B. (2013). Synthesis of 9-oxononanoic acid, a precursor for biopolymers. ChemSusChem, 6, 2149–2156.
  63. Kazenwadel, C., Klebensberger, J., Richter, S., Pfannstiel, J., Gerken, U., Pickel, B., … Hauer, B. (2013). Optimized expression of the dirigent protein AtDIR6 in Pichia pastoris and impact of glycosylation on protein structure and function. Appl Microbiol Biotechnol, 97, 7215–7227.
  64. Nestl, B., Nebel, B., & Hauer, B. (2013). Dirigent effects in biocatalysis. Protein Engineering Handbook, 3, 1–27.
  65. Hammer, S., Syren, P., Seitz, M., Nestl, B., & Hauer, B. (2013). Squalene hopene cyclases: highly promiscuous and evolable catalysts for stereoselective C-C and C-X bond formation. Curr. Opin. Chem. Biol., 17, 293–300.
  66. Scheps, D., Honda, M. S., Richter, S., Marisch, K., Nestl, B., & Hauer, B. (2013). Synthesis of omega-hydroxy dodecanoic acid based on an engineered CYP153A fusion construct. Microbial. Biotechnol., 6, 694–707.
  67. Vallon, T., Glemser, M., Honda, M. S., Scheps, D., Schmid, J., Siemann-Herzberg, M., … Takors, R. (2013). Production of 1-octanol from n-octane by Pseudomonas putida KT2440. Chemie Ingenieur Technik, 85, 841–848.
  68. Hammer, S., Nestl, B., & Hauer, B. (2013). The slumbering synthesis potential in enzymes: how can it be awakened? BIOspektrum, 19, 574–576.
  69. Zhong, L., Hauer, B., & Rosche, B. (2013). Catalytic biofilms on structured packing for the production of glycolic acid. J. Microbiol. Biotechnol., 23, 195–204.
  70. Mückschel, B., Simon, O., Klebensberger, J., Graf, N., Rosche, B., Altenbuchner, J., … Hauer, B. (2012). Ethylene Glycol Metabolism of Pseudomonas putida. Appl Environ Microbiol, 78, 8531–8539.
  71. Kazenwadel, C., Eiben, S., Maurer, S., Beuttler, H., Wetzl, D., Hauer, B., & Koschorreck, K. (2012). Thiol-functionalization of acrylic ester monomers catalyzed by immobilized Humicola insolens cutinase. Enzyme Microbiol. Technol., 51, 9–15.
  72. Seitz, M., Klebensberger, J., Siebenhaller, S., Breuer, M., Siedenburg, G., Jendrossek, D., & Hauer, B. (2012). Substrate specificity of a novel squalene-hopene cyclase from Zymomonas mobilis. J Mol Catal B: Enzym, 84, 72–77.
  73. Hammer, S., Dominicus, J., Syren, P., Nestl, B., & Hauer, B. (2012). Stereoselective Friedel-Crafts alkylation catalyzed by squalene hopene cyclases. Tetrahedron, 68, 7624–7629.
  74. Siedenburg, G., Jendrossek, D., Breuer, M., Juhl, B., Pleiss, J., Seitz, M., … Hauer, B. (2012). Activation-independent cyclization of monoterpenoids. Appl Environ Microbiol, 78, 1055–1062.
  75. Honda, M. S., Scheps, D., Kühnel, L., Venegas, E., Seifert, A., Nestl, B., & Hauer, B. (2012). Bacterial CYP153A monooxygenases for the synthesis of omega-hydroxylated fatty acids. Chem. Commun., 48, 5115–5117.
  76. Reich, S., Höffken, H., Rosche, B., Nestl, B., & Hauer, B. (2012). Crystal structure determination and mutagenesis analysis of the ene reductase NCR. ChemBioChem, 13, 2400–2407.
  77. Lalli, M., Facey, S., & Hauer, B. (2011). Protein Containers—Promising Tools for the Future. ChemBioChem, 12, 1519–1521.
  78. Beuttler, H., Hoffmann, J., Jeske, M., Hauer, B., Schmid, R., Altenbuchner, J., & Urlacher, V. (2011). Biosynthesis of zeaxanthin in recombinant Pseudomonas putida. Appl.Microbiol.Biotechnol., 89, 11377–11347.
  79. Scheps, D., Honda, M. S., Hoffmann, H., Nestl, B., & Hauer, B. (2011). Regioselective w-hydroxylation of medium-chain n-alkanes and primary alcohols by CYP153 enzymes from Mycobacterium marinum and Polaromonas sp. strain JS666. Org. Biomol. Chem., 9, 6727–6733.
  80. Nestl, B., Nebel, B., & Hauer, B. (2011). Recent progress in industrial biocatalysis. Curr. Opin. Chem. Biol, 15, 187–193.
  81. Alissandrotos, A., Baudendistel, N., Hauer, B., Baldenius, K., Flitsch, S., & Halling, P. (2011). Biocompatible functionalisation of starch. Chem.Commun., 47, 683–685.
  82. Seifert, A., Antonovici, M., Hauer, B., & Pleiss, J. (2011). An efficient route to selective bio-oxidation catalysts: an iterative approach comprising modeling, diversification, and screening, based on CYP102A1. ChemBioChem, 12, 1346–1351.
  83. Syren, P., Lindgre, E., Hoeffken, H., Branneby, C., Maurer, S., Hauer, B., & Hult, K. (2010). Increased activity of enzymatic transacylation of acrylates thorugh rational design of lipases. J Mol. Catal. B, 65, 3–10.
  84. Alissandrotos, A., Baudendistel, N., Flitsch, S., Hauer, B., & Halling, P. (2010). Lipase-catalysed acylation of starch and determination of the degree of substitution by methanolysis and GC. BMC Biotechnology, 10, 82.
  85. Güven, A., Fioroni, M., Hauer, B., & Schwaneberg, U. (2010). Molecular understanding of sterically controlled compound release through an engineered channel protein (FhuA). J. Nanotechnology, 8, 14.
  86. Koschorreck, K., Kazenwadel, C., Schmid, R., & Hauer, B. (2010). Heterologous expression, characterization and site-directed mutagenesis of cutinase CUTAB1 from Alternaria brassicicola. Appl. Microbiol. Biotechnol., 87, 991–997.
  87. Fisch, F., Fleites, C., Delenne, M., Baudendistel, N., Hauer, B., Turkenburg, J., … Grogan, G. (2010). A covalent succinylcysteine-like intermediate in the enzyme-catalyzed transformation of maleate ot fumerate by maleate isomerase. J. Am. Chem. Soc., 132, 11455–11457.
  88. Mueller, N., Stueckler, C., Hauer, B., Baudendistel, N., Housden, H., Bruce, N., & Faber, K. (2010). The Substrate Spectra of Pentaerythritol Tetranitrate Reductase, Morphinone Reductase, N-Ethylmaleimide Reductase and Estrogen-Binding Protein in the Asymmetric Bioreduction of Activated Alkenes. Adv. Synth. Catal, 325, 387–394.
  89. Roepcke, C., Muench, S., Schulze, H., Bachmann, T., Schmid, R., & Hauer, B. (2010). Analysis of phosphothionate pesticides using a chloroperoxidase pretreatment and acetylcholonesterase biosensor detection. J.Agric.Food Chem., 58, 8748–8756.
  90. Rosche, B., Hauer, B., Schmid, A., & Bühler, K. (2009). Microbial biofilms: a concept for industrial catalysis? Trends Biotechnol., 27(11), 636–643.
  91. Lentz, O., Feenstra, A., Habicher, T., Hauer, B., Schmid, R., & Urlacher, V. (2006). Altering the regioselectivity of cytochrome P450 CYP102A3 of Bacillus subtilis by using a new versatile assay system. Chembiochem, 7, 345–350.
  92. Doderer, K., Schmitt, J., Hauer, B., & Schmid, R. (2003). High-throughput screening for bacterial epoxide hydrolyase activity. Anal Biochem, 321, 131–134.
  93. Zocher, F., Enzelberger, M., Bornscheuer, U., Hauer, B., Wohlleben, W., & Schmid, R. (2000). Epoxide hydrolase activity of Streptomyces strains. J Biotechnol, 77, 287–292.
  94. Hauer, B., Schmid, R., Enzelberger, M., & Minning, S. (1999). Peptides for creation of fusion proteins and their purification by metal ion affinity chromatography. WO, 9960134.
  95. Zocher, F., Enzelberger, M., Bornscheuer, U., Hauer, B., & Schmid, R. (1999). A colorimetric assay suitable for screening epoxide hydrolase activity. Anal Chim Acta, 391, 345–351.
  96. Enzelberger, M., Zocher, F., Bornscheuer, U., Schmidt-Dannert, C., Hauer, B., & Schmid, R. (1998). Singularisation of enzyme mutants by use of a cell sorter and the green fluorescent protein. Bioforum, 21, 192–194.

AG Hauer



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