Abteilung Technische Biochemie

Publikationen

Hier finden Sie eine Gesamtübersicht der Publikationen der Abteilung.

Publikationen

  1. Buchholz P, Ohs R, Spieß A, Pleiss J. Progress curve analysis within BioCatNet: comparing kinetic models for enzyme-catalyzed self-ligation. Biotechnol J. 2019;14:e1800183.
  2. Grüninger M, Buchholz P, Mordhorst S, Strack P, Müller M, Hubrich F, et al. Chorismatases – the family is growing. Org Biomol Chem. 2019;17:2092–8.
  3. Gobeil S, Ebert M, Park J, Gagné D, Doucet N, Berghuis A, et al. The structural dynamics of engineered β-lactamases vary broadly on three timescales yet sustain native function. Sci Rep. 2019;9:6656–6656.
  4. Fries A, Mazzaferro L, Grüning B, Bisel P, Stibal K, Buchholz P, et al. Alteration of the route to menaquinone towards isochorismate - derived metabolites. ChemBioChem. 2019;
  5. Baz J, Held C, Pleiss J, Hansen N. Thermophysical properties of glyceline-water mixtures investigated by molecular modelling. Phys Chem Chem Phys. 2019;21:6467–76.
  6. Buchholz P, Ferrario V, Pohl M, Gardossi L, Pleiss J. Navigating within thiamine diphosphate-dependent decarboxylases: Sequences, structures, functional positions, and binding sites. Proteins. 2019;
  7. Schatz K, Krone M, Pleiss J, Ertl T. Interactive visualization of biomolecules’ dynamic and complex properties. Eur Phys J Special Topics. 2019;227:1725–39.
  8. Gräff M, Buchholz P, Stockinger P, Bommarius B, Bommarius A, Pleiss J. The Short-chain Dehydrogenase/Reductase Engineering Database (SDRED):  A classification and analysis system for a highly diverse enzyme family. Proteins. 2019;87:443–51.
  9. Demming R, Hammer S, Nestl B, Gergel S, Fademrecht S, Pleiss J, et al. Asymmetric Enzymatic Hydration of Unactivated, Aliphatic Alkenes. Angew Chem Int Ed Engl. 2019;58:173–7.
  10. Ferrario V, Pleiss J. Molecular simulations of enzymes under non-natural conditions. Eur Phys J Eur Phys J Special Topics. 2019;227:1631–8.
  11. Darimont D, Weissenborn M, Nebel B, Hauer B. Modulating proposed electron transfer pathways in P450BM3 led to improved activity and coupling efficiency. Bioelectrochemistry. 2018;119:119–23.
  12. Pleiss J. Thermodynamic activity-based progress curve analysis in enzyme kinetics. Trends Biotechnol. 2018;36:234–8.
  13. Wehrmann M, Klebensberger J. Engineering thermal stability and solvent tolerance of the soluble quinoprotein PedE from P. putida KT2440 with a heterologous whole-cell screening approach. Microb Biotechnol. 2018;11:399–408.
  14. Baierl A, Theorell A, Mackfeld U, Marquardt P, Hoffmann F, Moers S, et al. Towards a mechanistic understanding of factors controlling the stereoselectivity of transketolase. ChemCatChem. 2018;10:2601–11.
  15. Halder J, Nestl B, Hauer B. Semi-Rational Engineering of the Naphthalene Dioxygenase from Pseudomonas sp. NCIB 9816-4 towards Selective Asymmetric Dihydroxylation. ChemCatChem. 2018;10:178–82.
  16. Franden M, Jayakody L, Li W, Wagner N, Cleveland N, Michener W, et al. Engineering Pseudomonas putida KT2440 for efficient ethylene glycol utilization. Metab Eng. 2018;48:197–207.
  17. Martínez-Martínez M, Coscolín C, Santiago G, Chow J, Stogios P, ..., et al. Determinants and prediction of esterase substrate promiscuity patterns. ACS Chem Biol. 2018;13:225–34.
  18. Lenz M, Fademrecht S, Sharma M, Pleiss J, Grogan G, Nestl B. New imine-reducing enzymes from beta-hydroxyacid dehydrogenases by single amino acid substitutions. Protein Engineering, Design and Selection. 2018;31:109–20.
  19. Buß O, Buchholz P, Gräff M, Klausmann P, Rudat J, Pleiss J. The omega-Transaminase Engineering Database (oTAED): a navigation tool in protein sequence and structure space. Proteins. 2018;86:566–80.
  20. Lotti M, Pleiss J, Valero F, Ferrer P. Enzymatic production of biodiesel: strategies to overcome methanol inactivation. Biotechnol J. 2018;13:e1700155.
  21. Fernandes A, Halder J, Nestl B, Hauer B, Gernaey K, Krühne U. Biocatalyst Screening with a twist: Application of oxygen sensors integrated in microchannels for screening whole cell biocatalyst variants. Bioengineering. 2018;5:E30.
  22. Ferrario V, Hansen N, Pleiss J. Interpretation of cytochrome P450 monooxygenase kinetics by modeling of thermodynamic activity. J Inorg Biochem. 2018;183:172–8.
  23. Borlinghaus N, Nestl B. Switching the cofactor specificity of an imine reductase. ChemCatChem. 2018;10:183–7.
  24. Ferrario V, Pleiss J. Simulation of protein diffusion: a sensitive probe of protein-solvent interactions. J Biomol Struct Dyn. 2018;
  25. Buchholz P, Zeil C, Pleiss J. The scale-free nature of protein sequence space. PLoS One. 2018;13:e0200815.
  26. Borlinghaus N, Gergel S, Nestl B. Biocatalytic Access to Piperazines from Diamines and Dicarbonyls. ACS Catal. 2018;8:3727–32.
  27. Kress N, Halder J, Rapp L, Hauer B. Unlocked potential of dynamic elements in protein structures: channels and loops. Curr Opin Chem Biol. 2018;47:109–16.
  28. Demming R, Fischer M, Schmid J, Hauer B. (De)hydratases-recent developments and future perspectives. Curr Opin Chem Biol. 2018;43:43–50.
  29. Kühnel L, Nestl B, Hauer B. Enzymatic Addition of Alcohols to Terpenes by Squalene Hopene Cyclase Variants. ChemBioChem. 2017;18:2222–5.
  30. Wehrmann M, Billard P, Martin-Meriadec A, Zegeye A, Klebensberger J. Functional role of lanthanides in enzymatic activity and transcriptional regulation of PQQ-dependent alcohol dehydrogenases in Pseudomonas putida KT2440. mBIO. 2017;8:e00570-17.
  31. Pleiss J. Thermodynamic activity–based interpretation of enzyme kinetics. Trends Biotechnol. 2017;35:379–82.
  32. Bastian S, Hammer S, Kress N, Nestl B, Hauer B. Enabling New Selectivities in the Cyclization of Citronellal by Squalene Hopene Cyclase Variants. ChemCatChem. 2017;9:4364–8.
  33. Benson S, Pleiss J. Self-assembly nanostructures of triglyceride-water interfaces determine functional conformations of Candida antarctica lipase B. Langmuir. 2017;33:3151–9.
  34. Schmid J, Steiner L, Fademrecht S, Pleiss J, Otte K, Hauer B. Biocatalytic study of novel oleate hydratases. J Mol Catal B: Enzym. 2017;133:S243–9.
  35. Darimont D, Weissenborn M, Nebel B, Hauer B. Modulating proposed electron transfer pathways in P450BM3 led to improved activity and coupling efficiency. Bioelectrochemistry. 2017;119:119–23.
  36. Otte K, Maurer E, Kirtz M, Grabs D, Althoff E, Bartsch S, et al. Synthesis of sebacic acid using a de novo designed retro-aldolase as key catalyst. ChemCatChem. 2017;9:1378–82.
  37. Nestl B, Geinitz C, Popa S, Rizek S, Haselbeck R, Fischer M, et al. Structural and functional insights into asymmetric enzymatic dehydration of alkenols. Nat Chem Biol. 2017;13:275–81.
  38. Lenz M, Meisner J, Quertinmont L, Lutz S, Kästner J, Nestl B. Asymmetric Ketone Reduction by Imine Reductases. ChemBioChem. 2017;18:253–6.
  39. Zhang Q, Catti L, Pleiss J, Tiefenbacher K. Terpene cyclizations inside a supramolecular catalyst: Leaving-group-controlled product selectivity and mechanistic studies. J Am Chem Soc. 2017;139:11482–92.
  40. Klenk J, Nebel B, Porter J, Kulig J, Hussain S, Richter S, et al. The self-sufficient P450 RhF expressed in a whole cell system selectively catalyses the 5-hydroxylation of diclofenac. Biotechnol J. 2017;12:1600520.
  41. Krone M, Friess F, Scharnowski K, Reina G, Fademrecht S, Kulschewski T, et al. Molecular Surface Maps. IEEE Trans Vis Comput Graph. 2017;23:701–10.
  42. Demming R, Otte K, Nestl B, Hauer B. Optimized Reaction Conditions Enable the Hydration of Non-natural Substrates by the Oleate Hydratase from Elizabethkingia meningoseptica. ChemCatChem. 2017;9:758–66.
  43. Lenz M, Borlinghaus N, Weinmann L, Nestl B. Recent advances in imine reductase-catalzyed reactions. World J Microbiol Biotechnol. 2017;33:199.
  44. Buchholz P, Fademrecht S, Pleiss J. Percolation in protein sequence space. PLoS One. 2017;12:e0189646.
  45. Kress N, Rapp J, Hauer B. Enantioselective Reduction of Citral Isomers in NCR Ene Reductase: Analysis of an Active-Site Mutant Library. ChemBioChem. 2017;18:717–20.
  46. Hoffmann S, Danesh-Azari H-R, Spandolf C, Weissenborn M, Grogan G, Hauer B. Structure-guided redesign of CYP153AM.aq. for the improved terminal hydroxylation of fatty acids. ChemCatChem. 2016;8:3234–9.
  47. Weissenborn M, Notonier S, Lang S, Otte K, Herter S, Turner N, et al. Whole-cell microtiter plate screening assay for terminal hydroxylation of fatty acids by P450s. ChemCommun. 2016;52:6158–61.
  48. Gricman L, Weissenborn M, Hoffmann S, Borlinghaus N, Hauer B, Pleiss J. Redox Partner Interaction Sites in Cytochrome P450 Monooxygenases: In Silico Analysis and Experimental Validation. ChemistrySelect. 2016;6:1243–51.
  49. Kulschewski T, Pleiss J. Binding of solvent molecules to a protein surface in binary mixtures follows a competitive Langmuir model. Langmuir. 2016;32:8960–8.
  50. Schäfer I, Lasko G, Do T, Pleiss J, Weber U, Schmauder S. Peptide–zinc oxide interaction: Finite element simulation using cohesive zone models based on molecular dynamics simulation. Schmauder S SI, editor. Multiscale Materials Modeling. De Gruyter; 2016. (Schmauder S SI, editor. Multiscale Materials Modeling).
  51. Weissenborn M, Löw S, Borlinghaus N, Kuhn M, Kummer S, Rami F, et al. Enzyme-Catalyzed Carbonyl Olefination by the E. coli Protein YfeX in the Absence of Phosphines. ChemCatChem. 2016;
  52. Löw S, Löw I, Weissenborn M, Hauer B. Enhanced Ene-Reductase Activity through Alteration of Artificial Nicotinamide Cofactor Substituents. ChemCatChem. 2016;8:911–5.
  53. Bock S, Buchholz P, Vogel C, Holzapfel A, Pleiss J, Wiechert W, et al. Exploring the Sequence-Function Space of ThDP-Dependent Enzymes. Chemie Ingenieur Technik. 2016;88:1246.
  54. Wetzl D, Bolsinger J, Nestl B, Hauer B. α-Hydroxylation of Carboxylic Acids Catalyzed by Taurine Dioxygenase. ChemCatChem. 2016;8:1361–6.
  55. Pleiss J, Zeil C. Reply to The Curious Case of TEM-116. Antimicrob Agents Chemother. 2016;60:7001–7001.
  56. Moghimian P, Kilper S, Srot V, Rothenstein D, Facey SJ, Hauer B, et al. Phage-assisted assembly of organic–inorganic hybrid bilayers. International Journal of Materials Research. 2016;
  57. Fademrecht S, Scheller P, Nestl B, Hauer B, Pleiss J. Identification of imine reductase-specific sequence motifs. Proteins. 2016;84:600–10.
  58. Hammer S, Syren P, Hauer B. Substrate Pre-Folding and Water Molecule Organization Matters for Terpene Cyclase Catalyzed Conversion of Unnatural Substrates. Chemistry Select. 2016;1:3589–93.
  59. Kirtz M, Klebensberger J, Otte K, Richter S, Hauer B. Production of ω-hydroxy octanoic acid with Escherichia coli. J Biotechnol. 2016;230:30–3.
  60. Widmann M, Pleiss J. Sequence, structure, function: what we learn from analyzing protein families. A S, editor. Understanding enzymes. Singapore: pan Standord Publishing; 2016. (A S, editor. Understanding enzymes).
  61. Llaudet E, Darimont D, Samba R, Matiychyn I, Stelzle M, Weissenborn M, et al. Expanding an Efficient, Electrically Driven and CNT-Tagged P450 System into the Third Dimension: A Nanowired CNT-Containing and Enzyme-Stabilising 3 D Sol-Gel Electrode. ChemBioChem. 2016;17:1367–73.
  62. Syren P, Henche S, Eichler A, Nestl B, Hauer B. Squalene-hopene cyclases - evolution, dynamics and catalytic scope. Curr Opin Struct Biol. 2016;41:73–82.
  63. Hoffmann S, Weissenborn M, Gricman L, Notonier S, Pleiss J, Hauer B. The Impact of Linker Length on P450 Fusion Constructs: Activity, Stability and Coupling. ChemCatChem. 2016;8:1591–7.
  64. Buchholz P, Vogel C, Reusch W, Pohl M, Rother D, Spieß A, et al. BioCatNet: a database system for the integration of enzyme sequences and biocatalytic experiments. ChemBioChem. 2016;17:2093–8.
  65. Eichler A, Gricman L, Herter S, Kelly P, Turner N, Pleiss J, et al. Enantioselective benzylic hydroxylation catalysed by P450 monooxygenases: characterisation of a P450cam mutant library and molecular modelling. ChemBioChem. 2016;17:426–32.
  66. Benson S, Pleiss J. Computational modeling of a biocatalyst at a hydrophobic substrate interface. Nagel WE, Kröner DH RM, editor. High Performance Computing in Science and Engineering ´15. Springer International Publishing; 2016. (Nagel WE, Kröner DH RM, editor. High Performance Computing in Science and Engineering ´15).
  67. Reich S, Nestl B, Hauer B. Loop Grafted Old Yellow Enzymes in the Bienzymatic Cascade Reduction of Allylic Alcohols. ChemBioChem. 2016;17:561–5.
  68. Notonier S, Gricman L, Pleiss J, Hauer B. Semi-rational protein engineering of CYP153A M.aq.-CPR BM3 for efficient terminal hydroxylation of short to long chain fatty acids. ChemBioChem. 2016;17:1550–7.
  69. Jozwik I, Kiss F, Gricman L, Abdulmughni A, Brill E, Zapp J, et al. Structural basis of steroid binding and oxidation by the cytochrome P450 CYP109E1 from Bacillus megaterium. FEBS J. 2016;283:4128–48.
  70. Alqarni B, Colley B, Klebensberger J, McDougald D, Rice S. Expression stability of 13 housekeeping genes during carbon starvation of Pseudomonas aeruginosa. J Microbiol Meth. 2016;127:182–7.
  71. Zeil C, Widmann M, Fademrecht S, Vogel C, Pleiss J. Microdiversity of TEM β-lactamases: a network analysis of sequence-function relationships and exploration of sequence space. Antimicrob Agents Chemother. 2016;60:2709–17.
  72. Melcher M, Facey S, Henkes T, Subkowski T, Hauer B. Accelerated nucleation of hydroxyapatite using an engineered hydrophobin fusion protein. Biomacromolecules. 2016;
  73. Hinner L, Wissner J, Beurer A, Nebel B, Hauer B. Homogeneous vinyl ester-based synthesis of different cellulose derivatives in 1-ethyl-3-methyl-imidazolium acetate. Green Chemistry. 2016;
  74. Lenz M, Scheller P, Richter S, Hauer B, Nestl B. Cultivation and purification of two stereoselective imine reductases from Streptosporangium roseum and Paenibacillus elgii. Protein Expr Purif. 2016;
  75. Colley B, Dederer V, Carnell M, Kjelleberg S, Rice S, Klebensberger J. SiaA/D interconnects c-di-GMP and RsmA signaling to coordinate cellular aggregation of Pseudomonas aeruginosa in response to environmental conditions. Front Microbiol. 2016;7:179:doi: 10.3389/fmicb.2016.00179.
  76. Otte K, Hauer B. Enzyme engineering in the context of novel pathways and products. Curr Opin Biotechnol. 2015;35:16–22.
  77. Taudt A, Arnold A, Pleiss J. Simulation of protein association - Kinetic pathways towards crystal contacts. Phys Rev E. 2015;91:033311.
  78. Gricman L, Vogel C, Pleiss J. Identification of universal selectivity-determining positions in cytochrome P450 monooxygenases by systematic sequence-based literature mining. Proteins. 2015;83:1593-1603.
  79. Lundemo M, Notonier S, Striedner G, Hauer B, Woodley J. Process limitations of a whole-cell P450 catalyzed reaction using a CYP153A-CPR fusion construct expressed in Escherichia coli. Appl Microbiol Biotechnol. 2015;100:1197–208.
  80. Sasso F, Kulschewski T, Secundo F, Lotti M, Pleiss J. The effect of thermodynamic properties of solvent mixtures explains the difference between methanol and ethanol in C.antarctica lipase B catalyzed alcoholysis. J Biotechnol. 2015;214:1–8.
  81. Prins A, Kleinsmidt L, Khan N, Kirby B, Kudanga T, Vollmer J, et al. The effect of mutations near the T1 copper site on the biochemical characteristics of the small laccase from Streptomyces coelicolor A3(2). Enzyme Microb Technol. 2015;68:23–32.
  82. Löw S, Nestl B, Weissenborn M, Zepeck F, Hauer B. Process Investigations on the One-Pot Synthesis of Rifamycin S Avoiding Chlorinated Solvents. Org Process Res Dev. 2015;19:1544–7.
  83. Reznicek O, Facey SJ, Hauer B. Draft Genome Sequence of a Papaverine-Degrading, Gram-positive Arthrobacter sp., Isolated from Soil Near Hohenheim, Germany. Genome Announcements. 2015;3:e00422-15.
  84. Steudle A, Nestl B, Hauer B, Stubenrauch C. Activity of squalene-hopene cyclases in bicontinuous microemulsions. Colloids and Surfaces B: Biointerfaces. 2015;135:735–41.
  85. Gally C, Nestl B, Hauer B. Engineering Rieske Non-Heme Iron Oxygenases for the Asymmetric Dihydroxylation of Alkenes. Angew Chem Int Ed. 2015;54:12952–6.
  86. Hammer S, Marjanovic A, Dominicus J, Nestl B, Hauer B. Squalene Hopene Cyclases - Potency as Protonases for Stereoselective Brønsted Acid Catalysis. Nature Chemical Biology. 2015;11:121–6.
  87. Scheller P, Lenz M, Hammer S, Hauer B, Nestl B. Imine Reductase-Catalyzed Intermolecular Reductive Amination of Aldehydes and Ketones. ChemCatChem. 2015;7:3239–42.
  88. Felux A-K, Spiteller D, Klebensberger J, Schleheck D. Entner–Doudoroff pathway for sulfoquinovose degradation in Pseudomonas putida SQ1. Proc Natl Acad Sci U S A. 2015;112:E4298–305.
  89. Sandig B, Michalek L, Vlahovic S, Antonovici M, Hauer B, Buchmeiser M. A Monolithic Hybrid Cellulose-2.5-Acetate/Polymer Bioreactor for Biocatalysis under Continuous Liquid-Liquid Conditions Using a Supported Ionic Liquid Phase. Chemistry. 2015;21:15835–42.
  90. Lonsdale T, Lauterbach L, Honda MS, Nestl B, Hauer B, Lenz O. H2-driven biotransformation of n-octane to Q1 1-octanol by Q2 a recombinant Pseudomonas putida strain co-synthesizing an O2-tolerant hydrogenase and a P450 monooxygenase. Chem Commun. 2015;51:16173–5.
  91. Lotti M, Pleiss J, Valero F, Ferrer P. Effects of methanol on lipases: molecular, kinetic and process issues in the production of biodiesel. Biotechnol J. 2015;10:22–30.
  92. Simon O, Klebensberger J, Mückschel B, Kleiber I, Graf N, Altenbuchner J, et al. Analysis of the molecular response of Pseudomonas putida KT2440 to the next-generation biofuel n-butanol. J Proteomics. 2015;122:11–25.
  93. Reznicek O, Facey SJ, de WPP, Teunissen AWRH, de BJAM, Nijland JG, et al. Improved xylose uptake in Saccharomyces cerevisiae due to directed evolution of galactose permease Gal2 for sugar co-consumption. Journal of Applied Microbiology. 2015;119:99–111.
  94. Reznicek O, Luesken F, Facey SJ, Hauer B. Draft Genome Sequence of Phenylobacterium immobile Strain E (DSM 1986), Isolated from Uncontaminated Soil in Ecuador. Genome Announcements. 2015;3:e00420-15.
  95. Westphal R, Vogel C, Schmitz C, Pleiss J, Müller M, Pohl M, et al. A Tailor-Made Chimeric Thiamine Diphosphate Dependent Enzyme for the Direct Asymmetric Synthesis of (S)-Benzoins. Angew Chem Int Ed. 2014;53:9376–9.
  96. Schäfer I, Lasko G, Do T, Pleiss J, Weber U, Schmauder S. Peptide - zinc oxide interaction: finite element simulation using cohesive zone models based on molecular dynamics simulation. Comp Mater Sci. 2014;95:320–7.
  97. Westphal R, Jansen S, Vogel C, Pleiss J, Müller M, Rother D, et al. MenD from Bacillus subtilis: A Potent Catalyst for the Enantiocomplementary Asymmetric Synthesis of Functionalized α-Hydroxy Ketones. ChemCatChem. 2014;6:1082–8.
  98. Nestl B, Hauer B. Engineering of Flexible Loops in Enzymes. ACS Catalysis. 2014;4:3201–11.
  99. Widmann M, Pleiss J. Protein variants form a system of networks: microdiversity of IMP metallo-beta-lactamases. PLoS ONE. 2014;9:e101813.
  100. Syren P, Hammer S, Classen B, Hauer B. Entropy is Key to the Formation of Pentacyclic Terpenoids by Enzyme-Catalyzed Polycyclization. Angew Chem Int Ed. 2014;53:4845–9.
  101. Moghimian P, Srot V, Rothenstein D, Facey SJ, Harnau L, Hauer B, et al. Adsorption and Self-Assembly of M13 Phage into Directionally Organized Structures on C and SiO2 Films. Langmuir. 2014;30:11428–32.
  102. Baier J, Blumenstein N, Preusker J, Jeurgens L, Welzel U, Do T, et al. The influence of ZnO-binding 12-mer peptides on bio-inspired ZnO formation. Cryst Eng Comm. 2014;16:5301–7.
  103. Otte K, Kittelberg J, Kirtz M, Nestl B, Hauer B. Whole-Cell One-Pot Biosynthesis of Azelaic Acid. ChemCatChem. 2014;6,4:1003–9.
  104. Scharnowski K, Krone M, Reina G, Kulschewski T, Pleiss J, Ertl T. Comparative Visualization of Molecular Surfaces Using Deformable Models. Comput Graph Forum. 2014;3:191–200.
  105. Benson S, Pleiss J. Molecular dynamics simulations of self-emulsifying drug delivery systems (SEDDS): influence of excipients on droplet nanostructure and drug localization. Langmuir. 2014;30:8471–80.
  106. Pleiss J. Systematic analysis of large enzyme families: identification of specificity- and selectivity-determining hotspots. ChemCatChem. 2014;6:944–50.
  107. Reich S, Kress N, Nestl B, Hauer B. Variations in the stability of NCR ene reductase by rational enzyme loop modulation. J Struct Biol. 2014;185:228–33.
  108. Nebel B, Scheps D, Honda MS, Nestl B, Breuer M, Wagner H, et al. Biooxidation of n-butane to 1-butanol by engineered P450 monooxygenase under increased pressure. Journal of Biotechnology. 2014;191:86–92.
  109. Vogel C, Pleiss J. The modular structure of ThDP-dependent enzymes. Proteins. 2014;82:2523–37.
  110. Bogazkaya A, von BC, Kriening S, Busch A, Seifert A, Pleiss J, et al. Selective allylic hydroxylation of acyclic terpenoids by CYP154E1 from Thermobifida fusca YX. Beilstein J Org Chem. 2014;10:1347–53.
  111. Subinya M, Steudle A, Nestl B, Nebel B, Hauer B, Stubenrauch C, et al. Physico-chemical aspects of lipase B from Candida antarctica in bicontinuous microemulstions. Langmuir. 2014;30:2993–3000.
  112. Gricman L, Vogel C, Pleiss J. Conservation analysis of class-specific positions in cytochrome P450 monooxygenases: functional and structural relevance. Proteins. 2014;82:491–504.
  113. Ploss M, Facey SJ, Bruhn C, Zemel L, Hofmann K, Stark RW, et al. Selection of peptides binding to metallic borides by screening M13 phage display libraries. BMC Biotechnology. 2014;14:12.
  114. Benson S, Pleiss J. Solvent Flux Method (SFM): A case study of water access to Candida antarctica lipase B. J Chem Theory Comput. 2014;10:5206–14.
  115. Scheller P, Fademrecht S, Hofelzer S, Pleiss J, Leipold F, Turner N, et al. Enzyme Toolbox: Novel Enantiocomplementary Imine Reductases. ChemBioChem. 2014;15:2201–4.
  116. Nestl B, Hammer S, Nebel B, Hauer B. New Generation of Biocatalysts for Organic Synthesis. Angew Chem Int Ed. 2014;53:3070–95.
  117. Vallon T, Glemser M, Honda MS, Scheps D, Schmid J, Siemann-Herzberg M, et al. Production of 1-octanol from n-octane by Pseudomonas putida KT2440. Chemie Ingenieur Technik. 2013;85:841–8.
  118. Kulschewski T, Sasso F, Secundo F, Lotti M, Pleiss J. Molecular mechanism of deactivation of C. antarctica lipase B by methanol. J Biotechnol. 2013;168:462–9.
  119. Krone M, Reina G, Schulz C, Kulschewski T, Pleiss J, Ertl T. Interactive Extraction and Tracking of Biomolecular Surface Features. Comput Graph Forum. 2013;32:331–40.
  120. Zhong L, Hauer B, Rosche B. Catalytic biofilms on structured packing for the production of glycolic acid. J Microbiol Biotechnol. 2013;23:195–204.
  121. Benson S, Pleiss J. Incomplete mixing versus clathrate-like structures: a molecular view on hydrophobicity in methanol-water mixtures. J Mol Model. 2013;19:3427–36.
  122. Hammer S, Syren P, Seitz M, Nestl B, Hauer B. Squalene hopene cyclases: highly promiscuous and evolable catalysts for stereoselective C-C and C-X bond formation. Curr Opin Chem Biol. 2013;17:293–300.
  123. Hailes H, Rother D, Müller M, Westphal R, Ward J, Pleiss J, et al. Engineering stereoselectivity of ThDP-dependent enzymes. FEBS J. 2013;280:6374–94.
  124. Otte K, Kirtz M, Nestl B, Hauer B. Synthesis of 9-oxononanoic acid, a precursor for biopolymers. ChemSusChem. 2013;6:2149–56.
  125. Kulschewski T, Pleiss J. A molecular dynamics study of liquid aliphatic alcohols: simulation of density and self-diffusion coefficient using a modified OPLS force field. Mol Simulat. 2013;39:754–67.
  126. Roduner E, Kaim W, Sarkar B, Urlacher V, Pleiss J, Gläser R, et al. Selective catalytic oxidation of C–H bonds with molecular oxygen. ChemCatChem. 2013;5:82–112.
  127. Nestl B, Hauer B. Protein engineering: chemistry gets the assist. Nat Chem Bio. 2013;9:470–1.
  128. Nestl B, Nebel B, Hauer B. Dirigent effects in biocatalysis. Protein Engineering Handbook. 2013;3:1–27.
  129. Westphal R, Waltzer S, Mackfeld U, Widmann M, Pleiss J, Beigi M, et al. (S)-Selective MenD variants from Escherichia coli provide access to new functionalized chiral 2-hydroxy ketones. Chem Commun. 2013;49:2061–3.
  130. Scheps D, Honda MS, Richter S, Marisch K, Nestl B, Hauer B. Synthesis of omega-hydroxy dodecanoic acid based on an engineered CYP153A fusion construct. Microbial Biotechnol. 2013;6:694–707.
  131. Rothenstein D, Facey S, Ploss M, Hans P, Melcher M, Srot V, et al. Mineralisation of gold nanoparticles using tailored M13 phages. Bioinspired, Biomimetic and Nanobiomaterials. 2013;
  132. Hammer S, Nestl B, Hauer B. The slumbering synthesis potential in enzymes: how can it be awakened? BIOspektrum. 2013;19:574–6.
  133. Kazenwadel C, Klebensberger J, Richter S, Pfannstiel J, Gerken U, Pickel B, et al. Optimized expression of the dirigent protein AtDIR6 in Pichia pastoris and impact of glycosylation on protein structure and function. Appl Microbiol Biotechnol. 2013;97:7215–27.
  134. Seitz M, Syren P, Steiner L, Klebensberger J, Nestl B, Hauer B. Synthesis of heterocyclic terpenoids by promiscuous squalene-hopene cyclases. ChemBioChem. 2013;14:436–9.
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  184. Roepcke C, Muench S, Schulze H, Bachmann T, Schmid R, Hauer B. Analysis of phosphothionate pesticides using a chloroperoxidase pretreatment and acetylcholonesterase biosensor detection. JAgricFood Chem. 2010;58:8748–56.
  185. Mueller N, Stueckler C, Hauer B, Baudendistel N, Housden H, Bruce N, et al. The Substrate Spectra of Pentaerythritol Tetranitrate Reductase, Morphinone Reductase, N-Ethylmaleimide Reductase and Estrogen-Binding Protein in the Asymmetric Bioreduction of Activated Alkenes. Adv Synth Catal. 2010;325:387–94.
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  198. Koschorreck K, Schmid R, Urlacher V. Improving the functional expression of a Bacillus licheniformis laccase by random and site-directed mutagenesis. BMC Biotechnol. 2009;9:12–12.
  199. Klebensberger J, Birkenmaier A, Geffers R, Kjelleberg S, Philipp B. SiaA and SiaD are essential for inducing autoaggregation as a specific response to detergent stress in Pseudomonas aeruginosa. Environ Microbiol. 2009;11:3073–86.
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  207. Girhard M, Machida K, Itoh M, Schmid R, Arisawa A, Urlacher V. Regioselective biooxidation of (+)-valencene by recombinant E. coli expressing CYP109B1 from Bacillus subtilis in a two-liquid-phase system. Microb Cell Fact. 2009;8:36–36.
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  225. Soliman N, Knoll M, Abdel-Fattah Y, Schmid R, Lange S. Molecular cloning and characterization of thermostable esterase and lipase from Geobacillus thermoleovorans YN isolated from desert soil in Egypt. Process Biochem. 2007;42:1090–100.
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  230. Kühnel K, Maurer S, Galeyeva Y, Frey W, Laschat S, Urlacher V. Hydroxylation of dodecanoic acid and (2R,4R,6R,8R)-tetramethyldecanol on a preparative scale using an NADH-dependent CYP102A1 mutant. Adv Synth Catal. 2007;349 (8-9):1451–61.
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  234. Birkenmaier A, Holert J, Moeller H, Friemel A, Schoenenberger R, Suter M, et al. Biochemical and genetic investigation of initial reactions in aerobic degradation of the bile acid cholate in Pseudomonas sp. strain Chol1. J Bacteriol. 2007;189:7165–73.
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  237. Oelschlaeger P, Pleiss J. Hydroxyl groups in the betabeta sandwich of metallo-beta-lactamases favor enzyme activity: Tyr218 and Ser262 pull down the lid. J Mol Biol. 2007;366:316–29.
  238. Lange S, Richter S, Schmid R. High-level expression of extracellular lipase Lip2 from Yarrowia lipolytica in Pichia pastoris and its purification and characterization. Protein Expr Purif. 2007;53:255–63.
  239. Krause P, Singer E, Darley P, Klebensberger J, Groettrup M, Legler D. Prostaglandin E2 is a key factor for monocyte-derived dendritic cell maturation: enhanced T cell stimulatory capacity despite IDO. J Leuk Biol. 2007;82:1106–14.
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  259. Klebensberger J, Fritz E, Schink B, Philipp B. Cell aggregation of Pseudomonas aeruginosa strain PAO1 as an energy dependent stress response during growth with sodium dodecyl sulphate. Arch Microbiol. 2006;185:417–27.
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  261. Bachmann T, Leinberger D, Schumacher U, Autenrieth I. DNA-Chip für invasive Mykosen: Schnelle Analyse von Candida- und Aspergillus-Arten. Management und Krankenhaus. 2006;2:21–21.
  262. Knoll M, Müller M, Pleiss J, Pohl M. Factors mediating activity, selectivity, and substrate specificity for the thiamin diphosphate-dependent enzymes benzaldehyde lyase and benzoylformate decarboxylase. Chembiochem. 2006;7:1928–34.
  263. Zhang Y, Muench S, Schulze H, Perz R, Yang B, Schmid R, et al. Disposable biosensor test for organophosphate and carbamate insecticides in milk. J Agric Food Chem. 2005;29:5110–5.
  264. Dietrich M, Grundmann L, Kurr K, Valinotto L, Saussele T, Schmid R, et al. Recombinant production of human microsomal cytochrome P450 2D6 in the methylotrophic yeast Pichia pastoris. Chembiochem. 2005;6:2014–22.
  265. Maurer S, Schmid R. Biocatalysis for the epoxidation and hydroxylation of fatty acids and fatty alcohols. Hou CT, editor. Handbook of industrial biocatalysis, 2005. CRC Press LLC; 2005. 46 p. (Hou CT, editor. Handbook of industrial biocatalysis, 2005).
  266. Koschorreck M, Fischer M, Barth S, Pleiss J. How to find soluble proteins: a comprehensive analysis of alpha/beta hydrolases for recombinant expression in E. coli. BMC Genomics. 2005;6:1–10.
  267. Maurer S, Kühnel K, Kaysser L, Eiben S, Schmid R, Urlacher V. Catalytic hydroxylation in biphasic systems using CYP102A1 (P450 BM-3) mutants. Adv Synth Catal. 2005;347:1090–8.
  268. Mei L, Urlacher V, Schmid R. Cytochrome P450BM-3 mutants with improved catalytic properties of hydroxylating indole to indigo by error-prone PCR. Prog Biochem Biophys. 2005;32:630–5.
  269. Oelschlaeger P, Mayo S, Pleiss J. Impact of remote mutations on metallo-beta-lactamase substrate specificity: implications for the evolution of antibiotic resistance. Protein Sci. 2005;14:765–74.
  270. Bornscheuer U, Henke E, Pleiss J. Enzymatic methods. Christoffers, J.; Baro A, editor. Quaternary Stereocenters. Weinheim: Wiley-VCH; 2005. 13 p. (Christoffers, J.; Baro A, editor. Quaternary Stereocenters).
  271. Leinberger D, Schumacher U, Autenrieth I, Bachmann T. Development of a DNA microarray for the detection and identification of fungal pathogens involved in invasive mycoses. J Clin Microbiol. 2005;43:4943–53.
  272. Schulze H, Muench S, Villatte F, Schmid R, Bachmann T. Highly sensitive insecticide detection through protein engineering of Nippostrongylus brasiliensis acetylcholinesterase B. Anal Chem. 2005;77:5823–30.
  273. Bachmann T, Grimm V, Susa M, Knabbe C. DNA-Microarrays, Schneller Nachweis von ESBL verursachenden Laktamasen. Management und Krankenhaus. 2005;3:26–26.
  274. Schuster S, Enzelberger M, Trauthwein H, Schmid R, Urlacher V. pHluorin-based in vivo assay for hydrolase screening. Anal Chem. 2005;77:2727–32.
  275. Rusnak M, Nieveler J, Schmid R, Petri R. The putative lipase, AF1763, from Archaeoglobus fulgidusis a carboxylesterase with a very high pH optimum. Biotechnol Lett. 2005;11:743–8.
  276. Riepe F, Tatzel S, Sippell W, Pleiss J, Krone N. Congenital adrenal hyperplasia: the molecular basis of 21-hydroxylase deficiency in H-2(aw18) mice. Endocrinology. 2005;146:2563–74.
  277. Sulistyaningdyah W, Ogawa J, Maeda C, Yano Y, Schmid R, Shimizu S. Hydroxylation activity of P450 BM-3 mutant F87V towards aromatic compounds and its application to the synthesis of hydroquinone derivatives from phenolic compounds. Appl Microbiol Biotechnol. 2005;67:556–62.
  278. Schmid R. BioEngineering in China. Transkript. 2005;10:81–3.
  279. Karpushova A, Brümmer F, Barth S, Lange S, Schmid R. Cloning, recombinant expression and biochemical characterisation of novel esterases from Bacillus sp. associated with the marine sponge Aplysina aerophoba. Appl Microbiol Biotechnol. 2005;67:59–69.
  280. Villatte F, Kim B, Schmid R. In-gel patch electrophoresis: a new method for environmental DNA purification. Electrophoresis. 2005;26:3055–61.
  281. Appel D, Schmid R, Dragan C, Bureik M, Urlacher V. A fluorimetric assay for cortisol. Anal Bioanal Chem. 2005;383:182–6.
  282. Ogawa J, Schmid R, Shimizu S. Indole hydroxylation by bacterial cytochrome P450 BM-3 and modulation of activity by cumene hydroperoxide. Biosci Biotechnol Biochem. 2005;69:293–300.
  283. Villatte F, Schulze H, Schmid R, Bachmann T. Use of acetylcholinesterase and its modifications for biosensoring. Parveen, M.; Kumar S, editor. Recent Trends in the Acetylcholinesterase System. IOS Press; 2005. 17 p. (Parveen, M.; Kumar S, editor. Recent Trends in the Acetylcholinesterase System).
  284. Kauffmann I, Schmitt J, Schmid R. DNA isolation from soil samples for cloning in different hosts. Appl Microbiol Biotechnol. 2004;64:665–70.
  285. Urlacher V, Lutz-Wahl S, Schmid R. Microbial P450 enzymes in biotechnology. Appl Microbiol Biotechnol. 2004;64:317–25.
  286. Schulze H, Nonhoff U, Villatte F, Schmid R, Bachmann T. Highly sensitive neurotoxin detection using disposable acetylcholinesterase biosensors at elevated temperatures. Sensor Letters. 2004;2:121–4.
  287. Urlacher V, Schmid R. Protein engineering of the cytochrome P450 monooxygenase from Bacillus megaterium. Methods Enzymol. 2004;388:208–24.
  288. Susa M, Knabbe C, Schmid R, Bachmann T. Development and validation of a diagnostic DNA microarray to detect quinolone-resistant Escherichia coli among clinical isolates. J Clin Microbiol. 2004;42:4083–91.
  289. Grimm V, Ezaki S, Susa M, Knabbe C, Schmid R, Bachmann T. Use of DNA microarrays for rapid genotyping of TEM beta-lactamases that confer resistance. J Clin Microbiol. 2004;42:3766–74.
  290. Schulze H, Schmid R, Bachmann T. Activation of phosphorothionate pesticides based on a cytochrome P450 BM-3 (CYP102 A1) mutant for expanded neurotoxin detection in food using acetylcholinesterase biosensors. Anal Chem. 2004;76:1720–5.
  291. Doderer K, Schmid R. Fluorometric assay for determining epoxide hydrolase activity. Biotechnol Lett. 2004;26:835–9.
  292. Budde M, Maurer S, Schmid R, Urlacher V. Cloning, expression and characterisation of CYP102A2, a self-sufficient P450 monooxygenase from Bacillus subtilis. Appl Microb Biotech. 2004;66:180–6.
  293. Tejo B, Salleh A, Pleiss J. Structure and dynamics of Candida rugosa lipase: the role of organic solvent. J Mol Model. 2004;10:358–66.
  294. Lentz O, Urlacher V, Schmid R. Substrate specificity of native and mutated cytochrome P450 (CYP102A3) from Bacillus subtilis. J Biotechnol. 2004;108:41–9.
  295. Sulistyaningdyah W, Ogawa J, Shinkyo R, Sakaki T, Inouye K, Schmid R, et al. Metabolism of polychlorinated dibenzo-p-dioxins by cytochrome P450 BM-3 and its mutant. Biotechnol Lett. 2004;26:1857–60.
  296. Barth S, Fischer M, Schmid R, Pleiss J. Sequence and structure of epoxide hydrolases: a systematic analysis. Proteins. 2004;55:846–55.
  297. Blasco F, Kauffmann I, Schmid R. CYP175A1 from Thermus thermophilus HB27, the first beta-carotene hydroxylase of the P450 superfamily. Appl Microbiol Biotechnol. 2004;64:671–4.
  298. Strohmeier M, Hrmova M, Fischer M, Harvey A, Fincher G, Pleiss J. Molecular modeling of family GH16 glycoside hydrolases: potential roles for xyloglucan transglucosylases/hydrolases in cell wall modification in the poaceae. Protein Sci. 2004;13:3200–13.
  299. Schmid R, Pleiss J, Urlacher V. Biokatalyse: Selektivoxidation von C-H-Bindungen mit O2. Nachr Chem. 2004;52:767–72.
  300. Richter T, Muerdter T, Heinkele G, Pleiss J, Tatzel S, Schwab M, et al. Potent mechanism-based inhibition of human CYP2B6 by clopidogrel and ticlopidine. J Pharmacol Exp Ther. 2004;303:189–97.
  301. Barth S, Fischer M, Schmid R, Pleiss J. The database of epoxide hydrolases andhaloalkane dehalogenases: one structure, manyfunctions. Bioinformatics. 2004;20:2845–7.
  302. Currle-Linde N, Boes F, Lindner P, Pleiss J, Resch M. A Management System for Complex Parameter Studies and Experiments in Grid Computing. Gonzales T, editor. Proceedings of the 16th IASTED International Conference on PDCS - International Conference on Parallel and Distributed Computing and Systems. Cambridge: Acta press; 2004. 6 p. (Gonzales T, editor. Proceedings of the 16th IASTED International Conference on PDCS - International Conference on Parallel and Distributed Computing and Systems).
  303. Maurer S, Schulze H, Schmid R, Urlacher V. Immobilisation of P450 BM-3 and an NADP+ cofactor recycling system: towards a technical appication of heme-containing monooxygenases i fine chemical synthesis. Adv Synth Catal. 2003;345:802–10.
  304. Oelschlaeger P, Lange S, Schmitt J, Siemann M, Reuss M, Schmid R. Identification of factors impeding the production of a single-chain antibody fragment in Escherichia coli by comparing in vivo and in vitro expression. Appl Microbiol Biotechnol. 2003;61:123–32.
  305. Doderer K, Schmitt J, Hauer B, Schmid R. High-throughput screening for bacterial epoxide hydrolyase activity. Anal Biochem. 2003;321:131–4.
  306. Oelschlaeger P, Schmid R, Pleiss J. Insight into the mechanism of the IMP-1 metallo-beta-lactamase by molecular dynamics simulations. Protein Eng. 2003;16:341–50.
  307. Bachmann T. Transforming cyanobacteria into bioreporters of biological relevance. Trends Biotechnol. 2003;21:247–9.
  308. Schmid R, Lian M. Biowissenschaften in China. Nachr Chem. 2003;51:527–31.
  309. Henke E, Bornscheuer U, Schmid R, Pleiss J. A molecular mechanism of enantiorecognition of tertiary alcohols by carboxylesterases. ChemBioChem. 2003;4:485–93.
  310. Villatte F, Schulze H, Schmid R, Bachmann T. A long insertion reverts the functional effect of a substitution in acetylcholinesterase. Protein Eng. 2003;16:463–5.
  311. Oelschlaeger P, Schmid R, Pleiss J. Modeling domino effects in enzymes: molecular basis of the substrate specificity of the bacterial metallo-beta-lactamases IMP-1 and IMP-6. Biochemistry. 2003;42:8945–56.
  312. Schmid R. Chinese Bio Today. Chinese Bio Today.com. 2003;
  313. Fischer M, Pleiss J. The Lipase Engineering Database: a navigation and analysis tool for protein families. Nucleic Acids Res. 2003;31:319–21.
  314. Quyen DT, Schmidt-Dannert C, Schmid R. High-level expression of a lipase from Bacillus thermocatenulatus BTL2 in Pichia pastoris and some properties of the recombinant lipase. Protein Expr Purif. 2003;28:102–10.
  315. Lich N, Barth F, Pleiss J, Hai T. Homology modelling of alpha-amylase from Saccharomycopsis fibuligera with maltohexaose substrate. Vietnam Journal of Biotechnology. 2003;1:149–59.
  316. Fleming B, Tian Y, Bell S, Wong L, Urlacher V, Hill H. Redox properties of cytochrome P450BM3 measured by direct methods. Eur J Biochem. 2003;270:4082–8.
  317. Bessler C, Schmitt J, Maurer K, Schmid R. Directed evolution of a bacterial alpha-amylase: toward enhanced pH-performance and higher specific activity. Protein Sci. 2003;12:2141–9.
  318. Yano J, Blasco F, Schmid R, Henne A, Poulos T. Preliminary characterization and crystal structure of a thermostable cytochrome P450 from thermus thermophilus. J Biol Chem. 2003;278:608–16.
  319. Schulze H, Vorlova S, Villatte F, Bachmann T, Schmid R. Design of acetylcholinesterases for biosensor applications. Biosens Bioelectron. 2003;18:201–9.
  320. Schmid R. Pocket Guide to Biotechnology and Genetic Engineering. Schmid RD, editor. Pocket Guide to Biotechnology and Genetic Engineering. Winheim: Wiley VCH; 2003. 360 p. (Schmid RD, editor. Pocket Guide to Biotechnology and Genetic Engineering).
  321. Shumyantseva V, Bulko T, Usanov S, Schmid R, Nicolini C, Archakov A. Construction and characterization of bioelectrocatalytic sensors based on cytochromes P450. J Inorg Biochem. 2002;87:185–90.
  322. Urlacher V, Schmid R. Biotransformations using prokaryotic P450 monooxygenases. Curr Opin Biotechnol. 2002;13:557–64.
  323. Henke E, Pleiss J, Bornscheuer U. Activity of lipases and esterases towards tertiary alcohols: insights into structure-function relationships. Angew Chem Int Ed. 2002;41:3211–3.
  324. Bornscheuer U, Schmid R. Efficient water removal in lipase-catalyzed esterifications using a low-boiling-point azeotrope. Biotechnol Bioeng. 2002;78:31–4.
  325. Schulze H, Schmid R, Bachmann T. Rapid detection of neurotoxic insecticides in food using disposable acetyicholinesterase-biosensors and simple solvent extraction. Anal Bioanal Chem. 2002;372:268–72.
  326. Schlecht U, Nomura Y, Bachmann T, Karube I. Reversible surface thiol immobilization of carboxyl group containing haptens to a BIAcore biosensor chip enabling repeated usage of a single sensor surface. Bioconjug Chem. 2002;13:188–93.
  327. Gentner C, Schmid R, Pleiss J. Primary alcohols in a ring structure: quantifying enantioselectivity of Pseudomonas cepacia lipase by an in silico assay. Colloids Surf B Biointerfaces. 2002;26:57–66.
  328. Bathelt C, Schmid R, Pleiss J. Regioselectivity of CYP2B6: homology modeling, molecular dynamics simulation, docking. J Mol Model. 2002;8:327–35.
  329. Vorlova S, Bornscheuer U, Gatfield I, Hilmer J, Bertram H, Schmid R. Enantioselective Hydrolysis of d,l-Menthyl Benzoate to L-(-)-Menthol by Recombinant Candida rugosa Lipase LIP1. Adv Synth Catal. 2002;344:1152–5.
  330. Enzelberger M, Quake S. A nanoliter rotary device for polymerase chain reaction. Electrophoresis. 2002;23:1531–6.
  331. Kusharyoto W, Pleiss J, Bachmann T, Schmid R. Mapping of a hapten-binding site: molecular modeling and site-directed mutagenesis study of an anti-atrazine antibody. Protein Eng. 2002;15:233–41.
  332. Oelschlaeger P, Srikant-Iyer S, Lange S, Schmitt J, Schmid R. Fluorophor-linked immunosorbent assay: a time- and cost-saving method for the characterization of antibody fragments using a fusion protein of a single-chain antibody fragment and enhanced green fluorescent protein. Anal Biochem. 2002;309:27–34.
  333. Schulze H, Scherbaum E, Anastassiades M, Vorlova S, Schmid R, Bachmann T. Development, validation, and application of an acetylcholinesterase-biosensor test for the direct detection of insecticide residues in infant food. Biosens Bioelectron. 2002;17:1095–105.
  334. Villatte F, Bachmann T. How many genes encode cholinesterase in arthropods? Pesticide Biochem Physiol. 2002;73:122–9.
  335. Mbeunkui F, Richaud C, Etienne A, Schmid R, Bachmann T. Bioavailable nitrate detection in water by an immobilized luminescent cyanobacterial reporter strain. Appl Microbiol Biotechnol. 2002;60:306–12.
  336. Shumyantseva V, Bulko T, Schmid R, Archakov A. Photochemical properties of a riboflavins/cytochrome P450 2B4 complex. Biosens Bioelectron. 2002;17:233–8.
  337. Villatte F, Schulze H, Schmid R, Bachmann T. A disposable acetylcholinesterase-based electrode biosensor to detect anatoxin-a(s) in water. Anal Bioanal Chem. 2002;372:322–6.
  338. Schmitt J, Brocca S, Schmid R, Pleiss J. Blocking the tunnel: engineering of Candida rugosa lipase mutants with short chain length specificity. Protein Eng. 2002;15:595–601.
  339. Appel D, Lutz-Wahl S, Fischer P, Schwaneberg U, Schmid R. A P450 BM-3 mutant hydroxylates of alkanes, cycloalkanes, arenes and heteroarenes. J Biotechnology. 2001;88:167–71.
  340. Lange S, Schmitt J, Schmid R. High-level expression of the recombinant, atrazine-specific Fab fragment K411B by the methylotrophic yeast Pichia pastoris. J Immunol Methods. 2001;255:103–14.
  341. Minning S, Serrano A, Ferrer P, Sola C, Schmid R, Valero F. Optimization of the high-level production of Rhizopus oryzae lipase in Pichia pastoris. J Biotechnol. 2001;86:59–70.
  342. Villatte F, Bachmann T, Hussein A, Schmid R. Acetylcholinesterase assay for rapid expression screening in liquid and solid media. BioTechniques. 2001;30:81–6.
  343. Ogawa J, Schmid R, Shimizu S. Engineering cytochrome P450 BM-3 for oxidation of polycyclic aromatic hydrocarbons. Appl Environ Microbiol. 2001;67:5735–9.
  344. Musidlowska A, Lange S, Bornscheuer U. By overexpression in the yeast Pichia pastoris to enhanced enantioselectivity: New aspects in the application of pig liver esterase. Angew Chem Int Ed. 2001;40:2851–3.
  345. Lange S, Musidlowska A, Schmidt-Dannert C, Schmitt J, Bornscheuer U. Cloning, functional expression, and characterization of recombinant pig liver esterase. ChemBioChem. 2001;2:576–82.
  346. Lentz O, Schwaneberg U, Lutz-Wahl S, Fischer P, Schmid R. Modification of the fatty acid specificity of cytochrome P450 BM-3 from Bacillus megaterium by directed evolution: a validated assay. J Mol Catal B Enzym. 2001;15:123–33.
  347. Schwaneberg U, Fischer M, Schmitt J, Pleiss J, Lutz-Wahl S, Schmid R. Rational evolution of a medium chain-specific cytochrome P-450 BM-3 variant. Biochim Biophys Acta. 2001;1545:114–21.
  348. Schulz T, Schmid R, Pleiss J. Structural basis of stereoselectivity in Candida rugosa lipase-catalyzed hydrolysis of secondary alcohols. J Mol Model. 2001;7:265–70.
  349. Liu A, Somers N, Kazlauskas R, Brush T, Zocher F, Enzelberger M, et al. Mapping the substrate selectivity of new hydrolases using colorimetric screening: lipases from Bacillus thermocatenulatus and Ophiostoma piliferum, esterases from Pseudomonas fluorescens and Streptomyces diastatochromogenes. Biochimica et Biophysica Acta-Protein Structure and molecular Enzymology; Tetrahedron Asymmetry. 2001;12:545–56.
  350. Kahlow U, Schmid R, Pleiss J. A model of the pressure dependence of the enantioselectivity of Candida rugosa lipase towards (±)-menthol. Protein Sci. 2001;10:1942–52.
  351. Bornscheuer U, Stadler G, Lutz-Wahl S, Reuss M, Schmid R. Production of sugar fatty acid esters by enzymatic esterification in a stirred-tank membrane reactor: optimization of parameters by response surface methodology. J Am Oil Chem Soc. 2001;78:147–53.
  352. Schreiter P, Gillor O, Post A, Belkin S, Schmid R, Bachmann T. Monitoring of phosphorus bioavailability in water by an immobilized luminescent cyanobacterial reporter strain. Biosens Bioelectron. 2001;16:811–8.
  353. Baumeister F, Konig A, Schmid R, Kretschmer A, Bachmann T, Metzger J. Biosensors watching over the nitrification at wastewater treatment plants. Stuttgarter Berichte zur SiedlungswasserwirtschaftStickstoff im Wasser/Abwasser. München: Kommissionsverlag R. Oldenbourg; 2001. 11 p. (Stuttgarter Berichte zur SiedlungswasserwirtschaftStickstoff im Wasser/Abwasser).
  354. Ivanov Y, Kanaeva I, Gnedenko O, Pozdnev V, Shumyantseva V, Samenkova N, et al. Optical biosensor investigation of interactions of biomembrane and water-soluble cytochromes P450 and their redox partners with covalently immobilized phosphatidylethanolamine layers. J Mol Recognit. 2001;14:185–96.
  355. Bornscheuer U, Stadler G, Lutz-Wahl S, Otto R, Reuss M, Schmid R. Regioselective lipase-catalyzed synthesis of glucose ester on preparative scale. Eur J Lipid Sci Technol. 2001;103:583–7.
  356. Bachmann T, Pleiss J, Villatte F, Schmid R. Bioresponse-Linked Analysis Based on Acetylcholinesterase Inhibition. B H, editor. Bioresponse-Linked Instrumental Analysis. Stuttgart: B.G. Teubner; 2001. (B H, editor. Bioresponse-Linked Instrumental Analysis).
  357. Ogawa J, Schmid R, Shimizu S. Residue size at position 87 of cytochrome P450 BM-3 determines its stereoselectivity in propylbenzene and 3-chlorostyrene oxidation. FEBS Lett. 2001;508:249–52.
  358. Villatte F, Bachmann T, Hussein A, Schmid R. Expression level of heterologous proteins in Pichia pastoris is influenced by flasks design. Appl Microbiol Biotechnol. 2001;55:463–5.
  359. Traub P, Schmidt-Dannert C, Schmitt J, Schmid R. Gene synthesis, expression in E. coli, and in vitro refolding of Pseudomonas sp. KWI 56 and Chromobacterium viscosum lipases and their chaperones. Appl Microbiol Biotechnol. 2001;55:198–204.
  360. Musidlowska A, Lange S, Bornscheuer U. Durch Überexpression in der Hefe Pichia pastoris zu erhöhter Enantioselektivität: neue Aspekte bei der Anwendung von Schweineleber-Esterase. Angew Chem Int Ed. 2001;113:2934–6.
  361. Shumyantseva V, Bulko T, Bachmann T, Bilitewski U, Schmid R, Archakov A. Electrochemical reduction of flavocytochromes 2B4 and 1A2 and their catalytic activity. Arch Biochem Biophys. 2000;377:43–8.
  362. Peters H, Schmidt-Dannert C, Bornscheuer U, Schmid R. Purification and reconstitution of an integral membrane protein, the photoreaction center of Rhodobacter sphaeroides, using synthetic sugar esters. BioTechniques. 2000;28:1214–9.
  363. Pleiss J, Fischer M, Peiker M, Thiele C, Schmid R. Lipase Engineering Database: understanding and exploiting sequence-structure-function relationships. J Mol Catal B: Enzym. 2000;10:491–508.
  364. Enzelberger M, Minning S, Schmid R. Designing new metal affinity peptides by random mutagenesis of a natural metal-binding site. J Chromatogr A. 2000;898:83–94.
  365. Pleiss J, Scheib H, Schmid R. The his gap motif in microbial lipases: a determinant of stereoselectivity toward triacylglycerols and analogs. Biochimie. 2000;82:1043–52.
  366. Köhler S, Belkin S, Schmid R. Reporter gene bioassays in environmental analysis. Fesenius J Anal Chem. 2000;366:769–79.
  367. Zocher F, Enzelberger M, Bornscheuer U, Hauer B, Wohlleben W, Schmid R. Epoxide hydrolase activity of Streptomyces strains. J Biotechnol. 2000;77:287–92.
  368. Bornscheuer U, Pleiss J, Schmidt-Dannert C, Schmid R. Lipases from Rhizopus species: genetics, structure and applications. Alberghina L, editor. Protein engineering in industrial biotechnology. CRC Press; 2000. 20 p. (Alberghina L, editor. Protein engineering in industrial biotechnology).
  369. Schwaneberg U, Fischer P, Schmid R. Directed evolution of the fatty-acid hydroxylase P450 BM-3 into an indole-hydroxylating catalyst. Chemistry. 2000;6:1531–6.
  370. Bachmann T, Leca B, Villatte F, Marty J, Fournier D, Schmid R. Improved multianalyte detection of organophosphates and carbamates with disposable multielectrode biosensors using recombinant mutants of Drosophila acetylcholinesterase and artificial neural networks. Biosens Bioelectron. 2000;15:193–201.
  371. Pleiss J, Fischer M, Scheib H, Schulz T, Schmid R. Modelling structure-function relationship of lipase binding sites-a case study of specificity and selectivity. Kokotos G, editor. Lipases and LipidsStructure, function and biotechnological applications. Crete University Press; 2000. 19 p. (Kokotos G, editor. Lipases and LipidsStructure, function and biotechnological applications).
  372. Schulz T, Pleiss J, Schmid R. Stereoselectivity of Pseudomonas cepacia lipase towards secondary alcohols: a quantitative model. Protein Sci. 2000;9:1053–62.
  373. Köhler S, Bachmann T, Schmitt J, Belkin S, Schmid R. Detection of 4-chlorobenzoate using immobilized recombinant Escherichia coli reporter strains. Sens Actuators B. 2000;70:139–44.
  374. Pleiss J. In silico-Assays: Computergestuetzte Modellierung der Stereoselektivität von Lipasen. Transkript. 2000;9:38–40.
  375. Schwaneberg U, Appel D, Schmitt J, Schmid R. P450 in biotechnology: zinc driven w-hydroxylation of p-nitrophenoxydodecanoic acid using P450 BM-3 F87A as a catalyst. J Biotechnol. 2000;84:249–57.
  376. Otto R, Scheib H, Bornscheuer U, Pleiss J, Syldatk C, Schmid R. Substrate specificity of lipase B from Candida antarctica in the synthesis of arylaliphatic glycolipids. J Mol Catal B Enzym. 2000;8:201–11.
  377. Kovac A, Scheib H, Pleiss J, Schmid R, Paltauf F. Molecular basis of lipase stereoselectivity. Eur J Lipid Sci Tech. 2000;102:61–77.
  378. Hwang B, Scheib H, Pleiss J, Kim B, Schmid R. Computer-aided molecular modeling of the enantioselectivity of Pseudomonas cepacia lipase toward g- and d-lactones. J Mol Catal B Enzym. 2000;10:223–31.
  379. Pleiss J, Schmid R. Bioinformatics and the internet. Chemistry International. 1999;21:33–6.
  380. Quyen DT, Schmidt-Dannert C, Schmid R. High-level formation of active Pseudomonas cepacia lipase after heterolgous expression of the encoding gene and its modified chaperone in Escherichia coli and rapid in vitro refolding. Appl Environ Microbiol. 1999;65:787–94.
  381. Schmid U, Bornscheuer U, Soumanou M, McNeill G, Schmid R. Highly selective synthesis of 1,3-oleoyl-2-palmitoylglycerol by lipase catalysis. Biotechnol Bioeng. 1999;64:678–84.
  382. Soumanou M, Bornscheuer U, Schmid U, Schmid R. Crucial role of support and water activity on the lipase-catalyzed synthesis of structured triglycerides. Biocatal Biotransform. 1999;16:443–59.
  383. Zocher F, Enzelberger M, Bornscheuer U, Hauer B, Schmid R. A colorimetric assay suitable for screening epoxide hydrolase activity. Anal Chim Acta. 1999;391:345–51.
  384. Hauer B, Schmid R, Enzelberger M, Minning S. Peptides for creation of fusion proteins and their purification by metal ion affinity chromatography. WO. 1999;9960134.
  385. Bornscheuer U, Schmid R. Lipase-catalyzed synthesis of vitamin c fatty acid esters. Biotechnol Lett. 1999;21:1051–4.
  386. Scheib H, Pleiss J, Kovac A, Paltauf F, Schmid R. Stereoselectivity of Mucorales lipases toward triradylglycerols--a simple solution to a complex problem. Protein Sci. 1999;8:215–21.
  387. Pleiss J, Mionetto N, Schmid R. Probing the acyl binding site of acetylcholinesterase by protein engineering. J Mol Catal B: Enzym. 1999;6:287–96.
  388. Minning S, Weiss A, Bornscheuer U, Schmid R. Determination of peracid and putative enzymatic peracid formation by an easy colorimetric assay. Anal Chim Acta. 1999;378:293–8.
  389. Bornscheuer U, Schmid R. Lipase-catalyzed solid-phase synthesis of sugar esters. Influence of immobilization on productivity and stability of the enzyme. J Mol Catal B Enzym. 1999;6:279–85.
  390. Schwaneberg U, Sprauer A, Schmidt-Dannert C, Schmid R. P450 monooxygenase in biotechnology 1. Single-step, large-scale purification method for cytochrome P450 BM-3 by anion-exchange chromatography. J Chromatogr A. 1999;848:149–59.
  391. Cuong N, Bachmann T, Schmid R. Development of a dipstick immunoassay for quantitative determination of 2,4-dichlorophenoxyacetic acid in water, fruit and urine samples. Fresenius J Anal Chem. 1999;364:584–9.
  392. Bornscheuer U, Schmid R. Lipase-catalyzed solid-phase synthesis of sugar fatty acid esters. Removal of by-products by azeotropic distillation. Enzyme Microb Technol. 1999;25:725–8.
  393. Shumyantseva V, Bulko T, Alexandrova S, Sokolov N, Bachmann T, Schmid R, et al. N-terminal truncated cytochrome P450 2B4:  catalytic activities and reduction with alternative electron sources. Biochem Biophys Res Commun. 1999;263:678–80.
  394. Minning S, Scheib H, Traub P, Schmitt J, Schmid R. A toolbox of recombinant microbial lipases. Kokotos G, editor. Lipases and Lipids Structure, function and biotechnological applications Anwendungen. Santorini: Crete University Press; 1999. 20 p. (Kokotos G, editor. Lipases and Lipids Structure, function and biotechnological applications Anwendungen).
  395. Schwaneberg U, Schmidt-Dannert C, Schmitt J, Schmid R. A continuous spectrophotometric assay for P450 BM-3, a fatty acid hydroxylating enzyme, and its mutant F87A. Anal Biochem. 1999;269:359–66.
  396. Khalameyzer V, Fischer I, Bornscheuer U, Altenbuchner J. Screening, nucleotide sequence, and biochemical characterization of an esterase from Pseudomonas fluorescens with high activity towards lactones. Appl Environ Microbiol. 1999;65:477–82.
  397. McNiven S, Yano K, Ikebukuro K, Bornscheuer U, Schmid R, Karube I. Highly sensitive trilayer piezoelectric odor sensor. Anal Chim Acta. 1999;387:39–45.
  398. König A, Bachmann T, Metzger J, Schmid R. Disposable sensor for measuring the biochemical oxygen demand for nitrification and inhibition of nitrification in wastewater. Applied Microbiology and Biotechnology. 1999;51:112–7.
  399. Bachmann T, Schmid R. A disposable multielectrode biosensor for the discrimination of organophosphate and carbamate insecticides in the submicromolar range using native and recombinant variants of acetylcholinesterase. Anal Chim Acta. 1999;401:95–103.
  400. Beer H, McCarthy J, Bornscheuer U, Schmid R. Cloning, expression, characterization and role of the leader sequence of a lipase from Rhizopus oryzae. Biochim Biophys Acta. 1998;1399:173–80.
  401. Takahashi S, Ueda M, Atomi H, Beer H, Bornscheuer U, Schmid R, et al. Extracellular production of active Rhizopus oryzae lipase by Saccharomyces cerevisiae. J Ferment Bioeng. 1998;86:164–8.
  402. Soumanou M, Bornscheuer U, Schmid U, Schmid R. Synthesis of structured triglycerides by lipase catalysis. Fett/Lipid. 1998;100:156–60.
  403. Schmidt-Dannert C, Pleiss J, Schmid R. A toolbox of recombinant lipases for industrial applications. Ann N Y Acad Sci. 1998;864:14–22.
  404. Minning S, Schmidt-Dannert C, Schmid R. Functional expression of Rhizopus oryzae lipase in Pichia pastoris: high-level production and some properties. J Biotechnol. 1998;66:147–56.
  405. Bornscheuer U, Enzelberger M, Altenbuchner J. Mutator Strain Epicurian coli XL1-Red Generates Esterase Variants. Strategies Newsletter. 1998;11:16–7.
  406. Otto R, Bornscheuer U, Syldatk C, Schmid R. Lipase-catalyzed solid phase synthesis of 6-O-monoesters from beta-D(+)-glucose and aromatic acids. Synthesis. 1998;
  407. Schmid U, Bornscheuer U, Soumanou M, McNeill G, Schmid R. Optimization of the reaction conditions in the lipase-catalyzed synthesis of structured triglycerides. J Am Oil Chem Soc. 1998;75:1527–31.
  408. Schmid R, Verger R. Lipases: interfacial enzymes with attractive applications. Angew Chem Int Ed. 1998;37:1608–33.
  409. Bäumner A, Schmid R. Development of a new immunosensor for pesticide detection: a disposable system with lipsome-enhancement and amperometric detection. Biosens Bioelectron. 1998;13:519–29.
  410. Beimhorn D, Flemming H, Grummt T, Hansen P, Hock B, Kanne R, et al. Bioeffects-related environmental analytics. Part 1. Limits of chemical and biological analytics. Labor-Fachzeitschrift. 1998;42:905–8.
  411. Soumanou M, Schmid U, Bornscheuer U, Schmid R. Lipase-katalysierte Synthese von strukturierten Triglyceriden. Schriftenreihe. 1998;10:184–90.
  412. Beyersdorf-Radeck B, Riedel K, Karlson U, Bachmann T, Schmid R. Screening of xenobiotic compounds degrading microorganisms using biosensor techniques. Microbiol Res. 1998;153:239–45.
  413. Rua M, Atomi H, Schmidt-Dannert C, Schmid R. High-level expression of the thermoalkalophilic lipase from Bacillus thermocatenulatus in Escherichia coli. Appl Microbiol Biotechnol. 1998;49:405–10.
  414. Mosiello L, Segre L, Chiavarini S, Spano M, Cremisini C, Kimmel T, et al. Dipstick immunoassay format for atrazine and terbuthylazine analysis in water samples. J Agric Food Chem. 1998;46:3847–51.
  415. Mindermann A, Pleiss J, Hülser D. Molecular Modeling of Gap Junction Pores. Werner R, editor. Gap Junctions. Proceedings of the 8th International Gap Junction Conference, Key Largo, Florida. Florida: IOS Press; 1998. 5 p. (Werner R, editor. Gap Junctions. Proceedings of the 8th International Gap Junction Conference, Key Largo, Florida).
  416. Soumanou M, Bornscheuer U, Schmid R. Two-step enzymatic reaction for the synthesis of pure structured triacylglycerides. J Am Oil Chem Soc. 1998;75:703–10.
  417. Bornscheuer U, Schmid R. Lipase-catalyzed solid-phase synthesis of sugar esters, IV: Selectivity of lipases towards primary and secondary hydroxyl groups in carbohydrates. Biocatal Biotransform. 1998;16:249–57.
  418. Brocca S, Schmidt-Dannert C, Lotti M, Alberghina L, Schmid R. Design, total synthesis, and functional overexpression of the Candida rugosa lip1 gene coding for a major industrial lipase. Protein Sci. 1998;7:1415–22.
  419. Wagegg T, Enzelberger M, Bornscheuer U, Schmid R. The use of methoxy acetoxy esters significantly enhances reaction rates in the lipase-catalyzed preparation of enantiopure 1-(4-chloro phenyl) ethyl amines. J Biotechnol. 1998;61:75–8.
  420. Bachmann T, Bilitewski U, Schmid R. A microbial sensor based on Pseudomonas putida for phenol, benzoic acid and their monochlorinated derivatives which can be used in water and n-hexane. Anal Lett. 1998;31:2361–73.
  421. Hülser D, Eckert R, Irmer U, Krisciukaitis A, Mindermann A, Pleiss J, et al. Intercellular communication via gap junction channels. Bioelectrochem Bioenerg. 1998;45:55–65.
  422. Scheib H, Pleiss J, Stadler P, Kovac A, Potthoff A, Haalck L, et al. Rational design of Rhizopus oryzae lipase with modified stereoselectivity toward triradylglycerols. Protein Eng. 1998;11:675–82.
  423. Drees R, Pleiss J, Schmid R, Roller D. Integrating molecular modeling tools and virtual reality engines: an architecture for a highly immersive molecular modeling (HIMM) environment. Proceedings of CGI. 1998;391–2.
  424. Otto R, Bornscheuer U, Syldatk C, Schmid R. Synthesis of aromatic n-alkyl-glucoside esters in a coupled beta-glucosidase and lipase reaction. Biotechnology Letters. 1998;20:437–40.
  425. Heim J, Schmidt-Dannert C, Atomi H, Schmid R. Functional expression of a mammalian acetylcholinesterase in Pichia pastoris: comparison to acetylcholinesterase, expressed and reconstituted from Escherichia coli. Biochim Biophys Acta. 1998;1396:306–19.
  426. Pleiss J, Fischer M, Schmid R. Anatomy of lipase binding sites: the scissile fatty acid binding site. Chem Phys Lipids. 1998;93:67–80.
  427. Rubtsova M, Wittmann C, Egorov A, Schmid R. Chemiluminescent immunoassay: application of a portable scanning luminometer for the determination of 2,4-dichlorophenoxyacetic acid in microtiter and membrane strip format. Food Agric Immunol. 1998;9:235–47.
  428. Yano K, Karube I, Bornscheuer U, Schmid R. Development of an odorant sensor using polymer-coated quartz crystals modified with unusual lipids. Biosens Bioelectron. 1998;13:397–405.
  429. Rubtsova M, Egorov A, Schmid R. Simultaneous determination of several pesticides with chemiluminescent immunoassay on a multi-spot membrane strip. Food Agric Immunol. 1998;10:223–35.
  430. Lutz-Wahl S, Fischer P, Schmidt-Dannert C, Wohlleben W, Schmid R. Stereo- and regioselective hydroxylation of alpha-ionone by Streptomyces strains. Appl Environ Microbiol. 1998;64:3878–81.
  431. Otto R, Bornscheuer U, Scheib H, Pleiss J, Syldatk C, Schmid R. Lipase-catalyzed esterification of unusual substrates: synthesis of glucuronic acid and ascorbic acid. Biotechnol Lett. 1998;20:1091–4.
  432. Kisselev P, Wessel R, Pisch S, Bornscheuer U, Schmid R, Schwarz D. Branched phosphatidylcholines stimulate activity of cytochrome P450SCC (CYP11A1) in phospholipid vesicles by enhancing cholesterol binding, membrane incorporation and protein exchange. J Biol Chem. 1998;273:1380–6.
  433. Enzelberger M, Zocher F, Bornscheuer U, Schmidt-Dannert C, Hauer B, Schmid R. Singularisation of enzyme mutants by use of a cell sorter and the green fluorescent protein. Bioforum. 1998;21:192–4.
  434. Haalck L, Paltauf F, Pleiss J, Schmid R, Spener F, Stadler P. Stereoselectivity of lipase from Rhizopus oryzae toward triacylglycerols and anlogs: computer aided modelling and experimental validation. Methods Enzymol. 1997;284:353–76.
  435. Catoni E, Schmidt-Dannert C, Brocca S, Schmid R. Overexpression of lipase A and B of Geotrichum candidum in Pichia pastoris: high-level production and some properties of functional expressed lipase B. Biotechnol Tech. 1997;11:689–95.
  436. Yano K, Yoshitake H, Bornscheuer U, Schmid R, Ikebukuro K, Yokoyama K, et al. Development of a chemical vapor sensor using piezoelectric quartz crystals with coated unusual lipids. Anal Chim Acta. 1997;340:41–8.
  437. Fischer A, Bornscheuer U, Schmid R. Lipase-catalyzed solid phase synthesis of sugar fatty acid esters. Biocatalysis and Biotransformations. 1997;14:269–83.
  438. Wittmann C, Schmid R. Bioaffinity sensors for environmental monitoring. Kress-Rogers E, editor. Handbook of Biosensors and Electronic Noses: Medicine, Food and the Environment. Boca Raton/FL: CRC Press; 1997. 15 p. (Kress-Rogers E, editor. Handbook of Biosensors and Electronic Noses: Medicine, Food and the Environment).
  439. Rua M, Schmidt-Dannert C, Wahl S, Sprauer A, Schmid R. Thermoalkalophilic lipase of Bacillus thermocatenulatus large-scale production, purification and properties: aggregation behaviour and its effect on activity. J Biotechnol. 1997;56:89–102.
  440. Wittmann C, Riedel K, Schmid R. Microbial and enzyme sensors for environmental monitoring. Kress-Rogers E, editor. Handbook of Biosensors and Electronic Noses: Medicine, Food and the Environment. Boca Raton/FL: CRC Press; 1997. 34 p. (Kress-Rogers E, editor. Handbook of Biosensors and Electronic Noses: Medicine, Food and the Environment).
  441. Nishihara H, Okamura T, Schmid R, Hauck A, Reuss M. Biotechnological potential of P450 monooxygenases. High-level production of bovine cytochrome P450c17 monooxygenase during medium cell density culture of a recombinant yeast, Saccharomyces cerevisiae GRF 18 (YEp-Toku1). J Biotechnol. 1997;56:57–61.
  442. Pisch S, Bornscheuer U, Meyer M, Schmid R. Properties of unusual phospholipids .4. Chemoenzymatic synthesis of phospholipids bearing acetylenic fatty acids. Tetrahedron. 1997;53:14627–34.
  443. Soumanou M, Bornscheuer U, Menge U, Schmid R. Synthesis of structured triglycerides from peanut oil with immobilized lipase. J Am Oil Chem Soc. 1997;74:427–33.
  444. Schrag J, Cygler M, Lang D, Burgdorf T, Hecht H, Schmid R, et al. The open conformation of a Pseudomonas lipase. Structure. 1997;5:187–202.
  445. Kalisz H, Hendle J, Schmid R. Structural and biochemical properties of glycosylated and deglycosylated glucose oxidase from Penicillium amagasakiense. Appl Microbiol Biotechnol. 1997;47:502–7.
  446. Wittmann C, Schmid R, Löffler S, Zell A. Application of a neural network for pattern recognition of pesticides in water samples by different immunochemical techniques. Aga, D.S.; Thurman EM, editor. ACS Symposium Series No. 657, Immunochemical Technology for Environmental Applications. Oxford University Press; 1997. 18 p. (Aga, D.S.; Thurman EM, editor. ACS Symposium Series No. 657, Immunochemical Technology for Environmental Applications).
  447. Mionetto A, Morel N. Biochemical determination of insecticides via cholinesterases. 1. Acetylcholinesterase from rat brain: functional expression using a baculovirus system, and biochemical characterization. Biotechnol Tech. 1997;11:805–12.
  448. Pleiss J, Mionetto N, Schmid R. Protein engineering of rat brain acetylcholinesterase: a point mutation enhances sensitivityto pesticides. Protein Eng. 1997;10:66–66.
  449. Schmidt-Dannert C, Rua M, Wahl S, Schmid R. Bacillus thermocatenulatus lipase: a thermoalkalophilic lipase with interesting properties. Biochem Soc Trans. 1997;25:178–82.
  450. Enzelberger M, Bornscheuer U, Gatfield I, Schmid R. Lipase-catalysed resolution of gamma- and delta-lactones. J Biotechnol. 1997;56:129–33.
  451. Weber H, Zuegg J, Faber J, Pleiss J. Molecular reasons for lipase-sensitivity against acetaldehyde. J Mol Catal B: Enzym. 1997;3:131–8.
  452. Schmidt-Dannert C, Rua M, Schmid R. Two novel lipases from the thermophile Bacillus thermocatenulatus: cloning, overexpression in E. coli, purification and properties. Methods Enzymol. 1997;284:194–220.
  453. Schwarz D, Kisselev P, Wessel R, Pisch S, Bornscheuer U, Schmid R. Possible involvement of nonbilayer lipids in the stimulation of the activity of cytochrome P450SCC (CAP11A1) and its propensity to induce vesicle aggregation. Chem Phys Lipids. 1997;85:91–9.
  454. Holzwarth H-C, Pleiss J, Schmid R. Computer-aided modelling of stereoselective triglyceride hydrolysis catalyzed by Rhizopus oryzae lipase. J Mol Catal B Enzym. 1997;3:73–82.
  455. Schwarz D, Kisselev P, Pfeil W, Pisch S, Bornscheuer U, Schmid R. Evidence that nonbilayer phase propensity of the membrane is important for the side chain cleavage activity of cytochrome P450SCC. Biochemistry. 1997;36:14262–70.
  456. Peters H, Schmidt-Dannert C, Schmid R. The photoreaction center of Rhodobacter sphaeroides. Mater Sci Eng. 1997;C 4:227–32.
  457. Bäumner A, Kummer T, Schmid R. Liposome-based immunosensors. 1. Influence of hapten spacer length on liposome binding efficiency. Anal Lett. 1996;29:2601–13.
  458. Beer H, Wohlfahrt G, Schmid R, McCarthy J. The folding and activity of the extracellular lipase of Rhizopus oryzae are modulated by a prosequence. Biochem J. 1996;319:351–9.
  459. Wittmann C, Bilitewski U, Giersch T, Kettling U, Schmid R. Development and evaluation of a dipstick immunoassay format for the determination of atrazine residues on-site. Analyst. 1996;121:863–9.
  460. Gaziola L, Bornscheuer U, Schmid R. A rapid and effective separation of enantiomers of glycerol derivatives by gas chromatography and their lipase-catalyzed biotransformation. Enantiomer. 1996;1:49–54.
  461. Smidt H, Fischer A, Fischer P, Schmid R. Preparation of optically pure chiral amines by lipase-catalyzed enantioselective hydrolysis of N-acyl-amines. Biotechnol Tech. 1996;10:335–8.
  462. Schwarz D, Kisselev P, Wessel R, Jüptner O, Schmid R. Alpha-branched 1,2-diacyl phosphatidylcholines as effectors of activity of cytochrome P450SCC (CYP11A1). Modeling the structure of the fatty acyl chain region of cardiolipin. Journal of Biological Chemistry. 1996;271:12840–6.
  463. Beer H, Wohlfahrt G, McCarthy J, Schomburg D, Schmid R. Analysis of the catalytic mechanism of a fungal lipase using computer-aided design and structural mutants. Protein Eng. 1996;9:507–17.
  464. Bornscheuer U, Schmid R. Lipase-catalyzed solid-phase synthesis of sugar esters. Lipid/Fett. 1996;98:332–5.
  465. Heidt M, Bornscheuer U, Schmid R. Studies on the enantioselectivity in the lipase-catalyzed synthesis of  monoacylglycerols from isopropylidene glycerol. Biotechnol Tech. 1996;10:25–30.
  466. Schmidt-Dannert C, Rua M, Atomi H, Schmid R. Thermoalkalophilic lipase of Bacillus thermocatenulatus. I. molecular cloning, nucleotide sequence, purification and some properties. BBA-Lipid Lipid Met. 1996;1301:105–14.
  467. Bornscheuer U, Gaziola L, Schmid R. Application of vinyl esters for the lipase-catalyzed high-yield synthesis of monoacylglycerols. Ann NY Acad Sci. 1996;799:757–61.
  468. Lang D, Hofmann B, Haalck L, Hecht H, Spener F, Schmid R, et al. Crystal structure of a bacterial lipase from Chromobacterium viscosum ATCC 6918 refined at 1.6 angstroms resolution. J Mol Biol. 1996;259:704–17.
  469. Drees R, Pleiss J, Schmid R. Highly immersive molecular modeling (HIMM): an architecture for the integration of molecular modelling and virtual reality. Hofestädt R, Löffler M, Schomburg D, editors. Computer science and biology : proceedings of the German Conference on Bioinformatics (GCB 96). Leipzig: Universität Leipzig; 1996. 3 p. (Hofestädt R, Löffler M, Schomburg D, editors. Computer science and biology : proceedings of the German Conference on Bioinformatics (GCB 96)).
  470. Schmid R, Menge U, Schomburg D, Spener F. Towards novel biocatalysts via protein design: the case of lipases. FEMS Microbiol Rev. 1995;16:253–253.
  471. Westerhausen M, Enzelberger M, Schwarz W. Reaction of magnesium and calcium bisbis(trimethylsilyl)phosphanide with bisbis(trimethylsilyl)aminostannylene in a toluene solution. J Organomet Chem. 1995;491:83–90.
  472. Schmid R. In Vorbereitung auf große Wachstumsmärkte: Biotechnologie und Gentechnik in Japan, Korea, China und Taiwan. Pharmazeutische Industrie. 1995;57:370–9.
  473. Schmid R, Chung B, Jones A, Saono S, Scriven J, Tsai J. Biotechnology: a Multi-Volume Comprehensive Treatise. Rehm H, Reed G, Bauer D, editors. Modern Biotechnology: Legal, Economic and Ethical Dimensions. Weinheim: Wiley-VCH; 1995. 62 p. (Rehm H, Reed G, Bauer D, editors. Modern Biotechnology: Legal, Economic and Ethical Dimensions.).
  474. Rurup J, Mannova M, Brezesinki G, Schmid R. Properties of unusual phospholipids: I. Synthesis, monolayer investigations and calorimetry of diacylglycerophosphocholines containing monoacetylenic acyl chains. Chemistry and Physics of Lipids. 1994;70:187–98.
  475. Pleiss J. Quantitative modelling of lipase enantioselectivity. Svendsen A, editor. Enzyme functionality: Design, engineering, and screening. Marcel Dekker; 1994. (Svendsen A, editor. Enzyme functionality: Design, engineering, and screening).
  476. Pleiss J. Molecular basis of specificity and stereoselectivity of microbial lipases toward triacylglycerols. Bornscheuer UT, editor. Enzymes in Lipid Modification. Weinheim: Wiley-VCH; 1994. 15 p. (Bornscheuer UT, editor. Enzymes in Lipid Modification).
  477. Rurup J, Lauer F, Drewes A, Wray V, Schmid R. Properties of unusual phospholipids, II: Synthesis, NMR studies and monolayer investigations of diacylglycerophosphocholines containing alpha-branched acyl chains. Chem Phys Lipids. 1994;72:175–83.
  478. Pleiss J, Jähnig F. Conformational transition of an alpha-helix studied by molecular dynamics. Eur Biophys J. 1992;21:63–70.
  479. Pleiss J, Jähnig F. Collective vibrations of an alpha-helix. A molecular dynamics study. Biophys J. 1991;59:795–804.
  480. Roditi I, Schwarz H, Pearson T, Beecroft R, Liu M, Richardson J, et al. Procyclin gene expression and loss of the variant surface glycoprotein during differentiation of Trypanosoma brucei. J Cell Biol. 1989;108:737–46.
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