New publication in "PLOS ONE"

May 8, 2020 /

Design of synthetic tetracycline sensors

New publication in "PLOS ONE"

Antibiotics pollution in the environment is an important problem giving rise to the emergence of multi-resistant pathogens. In this work, we have established synthetic sensor system that can be established in E. coli cells to detect tetracycline and memorize its presence in form of DNA methylation patterns. Due to a positive feedback, the whole-cell biosensor showed a very high sensitivity for tetracycline. Moreover, acute presence of tetracycline and past exposure can be detected by this sensor using the dual readout of two reporter fluorophores.


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