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Institute of Biochemistry and Technical Biochemistry
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versity of Stuttgart
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Institute of Biochemistry and Technical Biochemistry
Department of Biochemistry
Department of Technical Biochemistry
Department of Food Chemistry
Department Biochemistry of cellular biomedical systems
Institute
Department of Biochemistry
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Publications by Prof. Jeltsch
Department Biochemistry of cellular biomedical systems
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Department of Food Chemistry
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Department of Technical Biochemistry
Research
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Publications
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Research
Kries Research Group
Bioinformatics
Bioinformatics
Designing aqueous deep eutectic mixtures by data-integrated simulation
PAZy a protein family database on plastics active enzymes
PyEED (Python Enzyme Engineering Database)
Understanding and designing substrate specificity of methyltransferases by molecular dynamics simulations and experiment
FAIR research data management in chemistry
EnzymeML - a data exchange format for biocatalysis and enzymology
Integration of sequence and reaction data for the design and engineering of SAM–dependent enzymes
FAIR research data management: Thermophysical properties and enzyme kinetics in deep eutectic solvents
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Department of Food Chemistry
Research
Equipment
Equipment
Mass spectrometer, chromatographic separation systems & other devices
Mass Spectrometer
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TripleTOF® 6600
High resolution QTOF mass spectrometer coupled to Micro-LC (Eksigent)
Ion source: ESI
Fragmentation mode: CID
Data acquisition: DDA, DIA, pseudoMRM
Application: bottom-up proteomics, lipidomics
Impact II™ (Bruker)
High resolution QTOF mass spectrometer coupled to UV/VIS-HPLC (Agilent)
Ion source: ESI
Fragmentation mode: CID
Data acquisition: DDA, pseudoMRM
Application: bottom-up proteomics, small molecules: DNA, polyphenols
amaZon ETD (Bruker)
Ion trap mass spectrometer coupled to UV/VIS-HPLC (Agilent)
Ion source: ESI
Fragmentation mode: CID, ETD, PTR
Data acquisition: DDA, MRM
n
Application: bottom-up proteomics, small molecules: amino acid analysis
Chromatographic Separation Systems
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AZURA® (Knauer)
Fast protein liquid chromatography (FPLC) coupled with UV/VIS detection
Application: peptide and protein purification
ÄKTA™ Purifier 10 (former GE Healthcare)
Fast protein liquid chromatography (FPLC) coupled with UV/VIS detection
Application: peptide and protein purification
HPLC 1260 (Agilent)
HPLC coupled with UV/VIS-Detector
Application: preparative separation of peptides, polyphenols and fruit acids
HPLC 1100 (Agilent)
Application: preparative separations
2 x HPLC LaChrom (Merck)
Application: preparative separations
Other Devices
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PAL DHR (CTC Analytics), Cooperation with Axel Semrau
Automated sample preparation
Automation platform: CHRONOS Software (Axel Semrau®)
Enables microwave assisted sample preparation in combination with Discover Proteomics (CEM)
Application: proteomics (in solution digest)
KingFisher™ Duo Prime (Thermo Fisher Scientific)
Automated purification system
Application: isolation and purification of aptamers and peptides by magnetic beads technology
Biometra TAdvanced (Analytik Jena)
PCR-Cycler
SPECTROstar Nano (BMG Labtech)
UV/Vis spectrometer with monochromator (220 - 1000 nm)
Application: concentration determination of proteins and polyphenols by absorption measurements in microplate and cuvette as well as enzyme assays
Infinite® 200 Pro (Tecan)
Filter-based multimode platereader for fluorescence and UV/VIS absorption measurements
Application: concentration determination of aptamers and peptides
BIO-5000 Plus (Serva)
VIS gel scanner
Application: scanning of electrophoresis gels by visual detection
BIO-1000F (Serva)
Fluorescence gel imager
Application: documentation of gels with fluorescent stain
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