Equipment

Mass spectrometer, chromatographic separation systems & other devices

Mass Spectrometer

  • High resolution QTOF mass spectrometer coupled to Micro-LC (Eksigent)
  • Ion source: ESI
  • Fragmentation mode: CID
  • Data acquisition: DDA, DIA, pseudoMRM
  • Application: bottom-up proteomics, lipidomics
  • High resolution QTOF mass spectrometer coupled to UV/VIS-HPLC (Agilent)
  • Ion source: ESI
  • Fragmentation mode: CID
  • Data acquisition: DDA, pseudoMRM
  • Application: bottom-up proteomics, small molecules: DNA, polyphenols
  • Ion trap mass spectrometer coupled to UV/VIS-HPLC (Agilent)
  • Ion source: ESI
  • Fragmentation mode: CID, ETD, PTR
  • Data acquisition: DDA, MRMn
  • Application: bottom-up proteomics, small molecules: amino acid analysis

Chromatographic Separation Systems

  • Fast protein liquid chromatography (FPLC) coupled with UV/VIS detection
  • Application: peptide and protein purification
  • Fast protein liquid chromatography (FPLC) coupled with UV/VIS detection
  • Application: peptide and protein purification
  • HPLC coupled with UV/VIS-Detector
  • Application: preparative separation of peptides, polyphenols and fruit acids
  • Application: preparative separations
  • Application: preparative separations

Other Devices

  • Automated sample preparation
  • Automation platform: CHRONOS Software (Axel Semrau®)
  • Enables microwave assisted sample preparation in combination with Discover Proteomics (CEM)
  • Application: proteomics (in solution digest)
  • Automated purification system
  • Application: isolation and purification of aptamers and peptides by magnetic beads technology
  • UV/Vis spectrometer with monochromator (220 - 1000 nm)
  • Application: concentration determination of proteins and polyphenols by absorption measurements in microplate and cuvette as well as enzyme assays
  • Filter-based multimode platereader for fluorescence and UV/VIS absorption measurements
  • Application: concentration determination of aptamers and peptides
  • VIS gel scanner
  • Application: scanning of electrophoresis gels by visual detection
  • Fluorescence gel imager
  • Application: documentation of gels with fluorescent stain
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