New publication in "J. Mol. Biol."

Methylation specific DNA binding domain (MBD) proteins are essential readers of DNA methylation in cells, but these proteins also have important biotechnological applications, which are described in this review article. MBD domains are used for global DNA methylation analysis, in vitro DNMT activity assays and to visualize methylated DNA in cells. Depending on the individual assay, MBD based detection systems for DNA methylation provide important advantages, ranging from cost efficiency and easy workflows to unique opportunities for the readout of methylation levels in living cells with locus-specific resolution during organismic development.