October 19, 2015 /

New publication in "Biochimie"

New data on structure and function of CcrM

DNA methylation at GANTC sites plays an essential role in the cell cycle of Caulobacter crescentus and related alpha-Proteobacteria, including several human pathogens. It is introduced by the Cell cycle regulated methyltransferase (CcrM). In this publication in Biochimie, we show that the CcrM contains a conserved C-terminal domain which is essential for activity. Mutation of residues in the CTD reduced activity and altered DNA binding. Our data suggest that the C-terminal domain is the so called “target recognition domain” of CcrM and based on this CcrM must be re-classified and it represents the first example of a delta-type DNA-(adenine N6)-methyltransferase.

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