Protein engineering of imine reductases

Data mining and molecular modelling for improving catalytic activity and substrate specificity of imine reductases.

The enzymatic reduction of imines is a promising approach to synthesize chiral amines. Therefore, biocatalysts with a broad substrate spectrum, high imine reductaseactivity, and high stereoselectivity are needed. Based on the Imine Reductase Engineering Database, we explore the sequence space of imine reductases to identify functionally relevant positions and to design variants with improved catalytic activity, extended substrate spectrum, and high stereoselectivity.


The enzymatic reduction of imines is a promising approach to synthesize chiral amines. Therefore, biocatalysts with a broad substrate spectrum, high imine reductaseactivity, and high stereoselectivity are needed. Based on the Imine Reductase Engineering Database, we explore the sequence space of imine reductases to identify functionally relevant positions and to design variants with improved catalytic activity, extended substrate spectrum, and high stereoselectivity.

Publications

    Project Members

    Peter Stockinger
     

    Peter Stockinger

    Bioinformatics

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