Protein engineering of imine reductases

Data mining and molecular modelling for improving catalytic activity and substrate specificity of imine reductases.

The enzymatic reduction of imines is a promising approach to synthesize chiral amines. Therefore, biocatalysts with a broad substrate spectrum, high imine reductaseactivity, and high stereoselectivity are needed. Based on the Imine Reductase Engineering Database, we explore the sequence space of imine reductases to identify functionally relevant positions and to design variants with improved catalytic activity, extended substrate spectrum, and high stereoselectivity.


The enzymatic reduction of imines is a promising approach to synthesize chiral amines. Therefore, biocatalysts with a broad substrate spectrum, high imine reductaseactivity, and high stereoselectivity are needed. Based on the Imine Reductase Engineering Database, we explore the sequence space of imine reductases to identify functionally relevant positions and to design variants with improved catalytic activity, extended substrate spectrum, and high stereoselectivity.

Publications

  1. Scheller, P., Fademrecht, S., Hofelzer, S., Pleiss, J., Leipold, F., Turner, N., … Hauer, B. (2014). Enzyme Toolbox: Novel Enantiocomplementary Imine Reductases. ChemBioChem, 15, 2201–2204.
  2. Fademrecht, S., Scheller, P., Nestl, B., Hauer, B., & Pleiss, J. (2016). Identification of imine reductase-specific sequence motifs. Proteins, 84, 600–610.

Project Members

Peter Stockinger
 

Peter Stockinger

Bioinformatics

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